Western Blotting (WB), ELISA, Immunoprecipitation (IP)
纯化方法
CD244 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
CD244 antibody was raised against a 12 amino acid synthetic peptide near the internal region of CD244.
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 75 kDa band observed in human Natural Killer (NK) cell lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1 kDa according to NP_057466.1). We do note that CD244 is a glycoprotein which makes a higher size band to be expected. Recommended concentration: 1-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.