CYBB antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
CYBB antibody was raised against a 13 amino acid synthetic peptide near the internal region of CYBB.
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 85Da band observed in human peripheral blood mononucleocytes ysates (calculated MW of 65.3 kDa according to NP_000388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Teahan et al, Nature. 1987 Jun 25-Jul 1,327 (6124) :720-1. PMID: 3600769). Recommended concentration: 0.03-0.1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.