Western Blotting (WB), Immunohistochemistry (IHC), ELISA
纯化方法
PPP4C antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
PPP4C antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of PPP4C.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 35 kDa band observed in HEK293, A431 and HeLa lysates (calculated MW of 35.1 kDa according to NP_002711.1). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human prostate shows nuclear staining of secretory cells in the gland. Recommended concentration, 2-4 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.