RAB25 抗体
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- 抗原 See all RAB25 抗体
- RAB25 (RAB25, Member RAS Oncogene Family (RAB25))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This RAB25 antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 纯化方法
- Antibody is purified by protein A affinity chromatography and resuspended in PBS with 0.2%BSA, 0.09% sodium azide.
- 免疫原
- Ni-NTA purified recombinant Rab25 expressed in E. Coli strain BL21 (DE3).
- 克隆位点
- 3F12F3
- 亚型
- IgG2a
- Top Product
- Discover our top product RAB25 Primary Antibody
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- 应用备注
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Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test. - 限制
- 仅限研究用
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- 缓冲液
- Purified antibody in PBS containing 0.03 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- 储存条件
- -20 °C
- 储存方法
- Rab25 monoclonal antibody can be stored at -20 °C, stable for one year.
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- 抗原
- RAB25 (RAB25, Member RAS Oncogene Family (RAB25))
- 别名
- Rab25 (RAB25 产品)
- 背景
- Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50 % similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40 % similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.
- 基因ID
- 57111
- UniProt
- P57735
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