AUH 抗体
Quick Overview for AUH 抗体 (ABIN2462112)
抗原
See all AUH 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Antibody is purified by peptide affinity chromatography method.
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免疫原
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AUH.
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anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (AA 68-339) antibody
AUH 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (AA 38-87) antibodyAUH 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (AA 44-135) antibodyAUH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (AA 228-277) antibodyAUH 适用: 人, 大鼠, 小鼠, Cow, 兔, 马, Pig, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibodyAUH 适用: 人, 大鼠, 小鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibodyAUH 适用: 人, 大鼠, 小鼠, 犬, Cow, 兔, 马, 豚鼠, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (AA 44-135) antibodyAUH 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (N-Term) antibodyAUH 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (N-Term) antibodyAUH 适用: 人, 大鼠, 小鼠, 犬, Cow, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- AUH antibody can be used for detection of AUH by ELISA at 1:312500. AUH antibody can be used for detection of AUH by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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注意事项
- As with any antibody avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store AUH antibody at -20 °C.
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- AUH (AU RNA Binding Protein/enoyl-CoA Hydratase (AUH))
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别名
- AUH
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背景
- AU-specific RNA-binding enoyl-CoA hydratase (AUH) protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92 % identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40- kDa precursor protein which is subsequently processed to a 32- kDa mature form.AU-specific RNA-binding enoyl-CoA hydratase (AUH) protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92 % identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40- kDa precursor protein which is subsequently processed to a 32- kDa mature form.
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分子量
- 37 kDa
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基因ID
- 549
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NCBI登录号
- NP_001689
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UniProt
- Q13825
抗原
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