NUP62 抗体 (Phenylalanine-Glycine Repeat)
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- 抗原 See all NUP62 抗体
- NUP62 (Nucleoporin 62kDa (NUP62))
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抗原表位
- AA 1-179, Phenylalanine-Glycine Repeat
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适用
- 人, 猴
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This NUP62 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 特异性
- Specific to human (HeLa cells) and simian (Cos cells). The antibody did not react with mouse.
- 纯化方法
- Purified from serum-free culture medium of the hybridoma by proprietary chromatography under mild conditions.
- 过滤
- Sterile filtered
- 免疫原
- Recombinant human Nup62 (amino acids 1-300) (GST-Nup62-His)
- 克隆位点
- 2A
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product NUP62 Primary Antibody
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- 应用备注
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1. Western blotting: 1/500 ~1/2,000 dilution
2. Immunoprecipitation (assay dependent)
3. Immunofluorescece / Immunocytochemistry: 1/400)
4. ELISA (assay dependent)
5. When this antibody was micro-injected into the cytoplasm of the HeLa cells, it accumulates into the nuclear pores as examined by immunofluorescence staining. . - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (x1), 50 % glycerol. Azide and carrier free.
- 储存液
- Azide free
- 储存条件
- -20 °C
- 储存方法
- Upon arrival centrifuge briefly and store at -20 C.
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Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies." in: Hybridoma (2005), Vol. 25, Issue 2, pp. 51-9, (2006) (PubMed).
: "Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins." in: Journal of cell science, Vol. 119, Issue Pt 21, pp. 4442-51, (2006) (PubMed).
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Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies." in: Hybridoma (2005), Vol. 25, Issue 2, pp. 51-9, (2006) (PubMed).
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- 抗原
- NUP62 (Nucleoporin 62kDa (NUP62))
- 别名
- Nup62 (NUP62 产品)
- 别名
- IBSN antibody, SNDI antibody, p62 antibody, NUP62 antibody, AA589433 antibody, AI426861 antibody, AI790512 antibody, AU045898 antibody, D7Ertd649e antibody, Nupc1 antibody, CG6251 antibody, Dmel\\CG6251 antibody, DDBDRAFT_0167902 antibody, DDBDRAFT_0237712 antibody, DDB_0167902 antibody, DDB_0237712 antibody, nup62 antibody, Np62 antibody, nucleoporin 62 antibody, hypothetical protein antibody, nucleoporin 62kDa antibody, Nucleoporin 62kD antibody, predicted protein antibody, nuclear pore protein antibody, Nup62 nuclear pore complex glycoprotein p62-related protein (IC) antibody, Nuclear pore glycoprotein p62 antibody, NUP62 antibody, PGTG_03077 antibody, Nup62 antibody, nup62 antibody
- 背景
- The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nucleoporins are the main components of the NPC in eukaryotic cells. Nup (Nucleoporin) 62 (522 aa, 53 kDa) is a member of the FG-repeat containing nucleoporins and is localized to the NPC central plug. Nup62 associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Predicted to contain about 10 N-acetylglucosamine side chain .
- UniProt
- P37198
- 途径
- EGFR Signaling Pathway, SARS-CoV-2 Protein Interactome
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