NUP153 抗体 (AA 610-1191)
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- 抗原 See all NUP153 抗体
- NUP153 (Nucleoporin 153kDa (NUP153))
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抗原表位
- AA 610-1191
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适用
- 大鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This NUP153 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Specific to human, mouse, rat and monkey Nup153 proteins.
- 交叉反应 (详细)
- Not tested for other species
- 产品特性
- When injected into the nucleus,R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well.
- 纯化方法
- Purified
- 过滤
- Sterile filtered
- 免疫原
- Recombinant GST-fused rat Nup153 (610-1191aa, Zn finger and FG repeats domain)
- 克隆位点
- R4C8
- 亚型
- IgG2a kappa
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- 应用备注
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1. Western blotting: 160 kDa single band in Hela cell extract
2. Immunocytochemistry
3. ELISA
Other applications have not been tested.
Additional comments:
When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells.
R4C8 works in immuno-cytochemistry very well. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 50 % glycerol
- 储存液
- Azide free
- 储存条件
- -20 °C
- 储存方法
- Upon receipt aliquot and store at -20 C
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Nanomechanical basis of selective gating by the nuclear pore complex." in: Science (New York, N.Y.), Vol. 318, Issue 5850, pp. 640-3, (2007) (PubMed).
: "The nucleoporin nup153 plays a critical role in multiple types of nuclear export." in: Molecular biology of the cell, Vol. 10, Issue 3, pp. 649-64, (1999) (PubMed).
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Nanomechanical basis of selective gating by the nuclear pore complex." in: Science (New York, N.Y.), Vol. 318, Issue 5850, pp. 640-3, (2007) (PubMed).
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- 抗原
- NUP153 (Nucleoporin 153kDa (NUP153))
- 别名
- Nup153 (NUP153 产品)
- 背景
- The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153 kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.
- UniProt
- P49790
- 途径
- Protein targeting to Nucleus
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