HIV-1 p24 抗体 (Biotin)
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- 抗原 See all HIV-1 p24 抗体
- HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))
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适用
- Human Immunodeficiency Virus (HIV)
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HIV-1 p24 antibody is conjugated to Biotin
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应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
- 产品特性
- This product is a biotinylated IgG ([biotin]/[IgG] = 8.0) produced from the purified IgG fraction of rabbit anti-p24 serum.
- 纯化方法
- Purified
- 过滤
- Sterile filtered
- 亚型
- IgG
- Top Product
- Discover our top product HIV-1 p24 Primary Antibody
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- 应用备注
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1. Western blotting
2. Immunoprecipitaiton
3. Immunofluorescence staining
4. ELISA
Not tested in other application - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.9 mg/mL
- 缓冲液
- PBS, 50 % glycerol
- 储存液
- Azide free
- 储存条件
- -20 °C
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HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).
: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).
: "A simple method for overproduction and purification of p24 Gag protein of human immunodeficiency virus type 1." in: Microbiology and immunology, Vol. 36, Issue 8, pp. 823-31, (1993) (PubMed).
: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).
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HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).
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- 抗原
- HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))
- 别名
- HIV-1 p24 (HIV-1 p24 产品)
- 物质类
- Viral Protein
- 背景
- HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction. p24 is used as a marker antigen for observing the patient's condition after treatment, as it indicates the amount of virus in the blood. The product is prepared by immunizing rabbit with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus, subtype B, and was highly purified by several steps of chromatography. Using this antiserum in Western blotting, the bands of 24 kD, 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells.
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