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5-Methylcytosine 抗体

IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD unconjugated
产品编号 ABIN2451915
发货至: 中国
  • 抗原
    5-Methylcytosine
    适用
    请咨询
    宿主
    • 22
    • 8
    • 3
    小鼠
    克隆类型
    • 24
    • 9
    单克隆
    标记
    • 16
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    非结合性
    应用范围
    • 20
    • 16
    • 13
    • 10
    • 8
    • 8
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC)
    纯化方法
    Purified
    过滤
    Sterile filtered
    免疫原
    5-Methylcytosine conjugated to bovine serum albumin
    克隆位点
    5MC-CD
    亚型
    IgM
  • 应用备注
    1) Immunocytochemistry: ~50-100 fold dilution
    2) Immuno-blotting detection of DNA with 5-methylcytosine on nitrocellulose: ~1000 fold dilution
    Not tested with other application
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS with 50 % Glycerol
    储存液
    Azide free
    储存条件
    -20 °C/-80 °C
    储存方法
    -20 C (For long term storage: -80 C)
  • Sharif, Muto, Takebayashi, Suetake, Iwamatsu, Endo, Shinga, Mizutani-Koseki, Toyoda, Okamura, Tajima, Mitsuya, Okano, Koseki: "The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA." in: Nature, Vol. 450, Issue 7171, pp. 908-12, (2007) (PubMed).

    Nishiyama, Ito, Yamaguchi, Koizumi, Sano: "A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 9, pp. 5925-30, (2002) (PubMed).

    Sano, Imokawa, Sager: "Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine." in: Biochimica et biophysica acta, Vol. 951, Issue 1, pp. 157-65, (1988) (PubMed).

    Sano, Royer, Sager: "Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, Issue 6, pp. 3581-5, (1980) (PubMed).

  • 抗原
    5-Methylcytosine
    别名
    5 Methylcytosine
    物质类
    Chemical
    背景
    DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA - for example, to the number 5 carbon of the cytosine pyrimidine ring - which involves reduction in gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context, non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.
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