Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
序列
YPYDVPDYA
特异性
Recognizes HA tagged (YPYDVPDYA) proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
纯化方法
Protein A purified
免疫原
Synthetic peptide containing the sequence aa98-106 (YPYDVPDYA) derived from the influenza virus hemaglutinin (HA) protein (KLH).
Type of Immunogen: Synthetic peptide - KLH conjugated
Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 0.5-2 μg/mL. Detects recombinant protein containing the HA-tag at the N-terminus in RIPA lysates from HEK293 cells. Immunoprecipitation: 5 μg/mL. Reported to immunoprecipitate recombinant HA-tagged fusion protein. Immunocytochemistry: 5 μg/mL. Reported to immunostain HA-tagged fusion protein.
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
PBS, pH 7.4, 0.05 % sodium azide, 40 % glycerol.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeat freeze-thaw cycles.
储存条件
4 °C,-20 °C
储存方法
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.