GFP 抗体
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- 抗原 See all GFP 抗体
- GFP (Green Fluorescent Protein (GFP))
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适用
- Aequorea victoria
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GFP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Recognizes Green Fluorescent Protein (GFP). Species cross-reactivity: all variants of Aequorea victoria GFP, S65T-GFP, RS-GFP, YFP and EGFP. Detects 5 ng of recombinant GFP (ECL) with 1 minute exposure or less.
- 纯化方法
- Affinity purified
- 免疫原
- Highly purified recombinant GFP made in E. coli.
- 亚型
- IgG
- Top Product
- Discover our top product GFP Primary Antibody
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
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- 应用备注
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Approved: ICC (1:200 - 1:1000), IF, IHC, IHC-P (1:2500 - 1:10000), IP, WB (1:2500 - 1:10000)
Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry, Immunohistochemistry, Immunofluorescence and Immunogold Electron Microscopy. Western Blot: 1:2500-1:10000 in 1X BLOTTO or 3 % BSA in PBS. Detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins. Immunocytochemistry: 1:200-1:1000 in 4 % normal serum. Very good signal on recombinant YES-GFP chimeras expressed in COS cells. Immunohistochemistry: 1:200 with HRP detection systems. Has been shown to be useful on mouse sections fixed with formalin. Immunohistochemistry: 1:200 for paraffin sections. Requires heat mediated antigen retrieval via the microwave method before IHC staining (13 min in 10 mM citrate buffer pH 6 followed by rapid cooling on ice). Immunoprecipitation: 1 μL per 10cm tissue culture dish of transfected cells. Use 10 μL protein A agarose CL4B to precipitate immune complex. Immunogold Electron Microscopy: 1:100. - 说明
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Target Species of Antibody: Aequorea victoria
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.2, 0.01 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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