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WISP1 抗体 (C-Term)

Verified WISP1 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN238610
发货至: 中国
  • 抗原 See all WISP1 抗体
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    抗原表位
    • 15
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 40
    • 26
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 55
    • 5
    • 1
    山羊
    克隆类型
    • 57
    • 4
    多克隆
    标记
    • 26
    • 9
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This WISP1 antibody is un-conjugated
    应用范围
    • 35
    • 21
    • 19
    • 13
    • 13
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    WISP1
    序列
    ESYPDFSEIA N
    特异性
    This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1).
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1, NP_543028.1.
    亚型
    IgG
  • 应用备注

    Western Blot: Approx 32-35 kDa band observed in lysates of cell line CaCo-2 and approx. 40 kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    别名
    CCN4 (WISP1 产品)
    别名
    CCN4 antibody, WISP1c antibody, WISP1i antibody, WISP1tc antibody, WISP-1 antibody, WISP1 antibody, si:ch211-38f10.2 antibody, AW146261 antibody, Elm1 antibody, zgc:172276 antibody, WNT1 inducible signaling pathway protein 1 antibody, WNT1 inducible signaling pathway protein 1a antibody, WNT1-inducible-signaling pathway protein 1-like antibody, WNT1 inducible signaling pathway protein 1b antibody, WISP1 antibody, wisp1a antibody, Wisp1 antibody, LOC100627044 antibody, wisp1b antibody
    背景
    WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1
    基因ID
    8840, 22402, 65154
    NCBI登录号
    NP_003873, NP_543028
    途径
    WNT signaling, Growth Factor Binding
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