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WISP1 抗体 (C-Term)

Verified WISP1 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN238610
发货至: 中国
  • 抗原 See all WISP1 抗体
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    抗原表位
    • 15
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 40
    • 26
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 55
    • 5
    • 1
    山羊
    克隆类型
    • 57
    • 4
    多克隆
    标记
    • 26
    • 9
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This WISP1 antibody is un-conjugated
    应用范围
    • 35
    • 21
    • 19
    • 13
    • 13
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    WISP1
    序列
    ESYPDFSEIA N
    特异性
    This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1).
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1, NP_543028.1.
    亚型
    IgG
  • 应用备注

    Western Blot: Approx 32-35 kDa band observed in lysates of cell line CaCo-2 and approx. 40 kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    别名
    CCN4 (WISP1 产品)
    背景
    WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1
    基因ID
    8840, 22402, 65154
    NCBI登录号
    NP_003873, NP_543028
    途径
    WNT signaling, Growth Factor Binding
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