WISP1 抗体 (C-Term)
-
- 抗原 See all WISP1 抗体
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
-
抗原表位
- C-Term
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This WISP1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- WISP1
- 序列
- ESYPDFSEIA N
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1).
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1, NP_543028.1.
- 亚型
- IgG
-
-
- 应用备注
-
Western Blot: Approx 32-35 kDa band observed in lysates of cell line CaCo-2 and approx. 40 kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
-
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
- 别名
- CCN4 (WISP1 产品)
- 别名
- CCN4 antibody, WISP1c antibody, WISP1i antibody, WISP1tc antibody, WISP-1 antibody, WISP1 antibody, si:ch211-38f10.2 antibody, AW146261 antibody, Elm1 antibody, zgc:172276 antibody, WNT1 inducible signaling pathway protein 1 antibody, WNT1 inducible signaling pathway protein 1a antibody, WNT1-inducible-signaling pathway protein 1-like antibody, WNT1 inducible signaling pathway protein 1b antibody, WISP1 antibody, wisp1a antibody, Wisp1 antibody, LOC100627044 antibody, wisp1b antibody
- 背景
- WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1
- 基因ID
- 8840, 22402, 65154
- NCBI登录号
- NP_003873, NP_543028
- 途径
- WNT signaling, Growth Factor Binding
-