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CK1 epsilon 抗体 (C-Term)

Name: CK1 epsilon 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN238540
Availability: OutOfStock

Verified CSNK1E 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated

ABIN238540 (1 μg/mL) staining of A431(A), HeLa (B) and K562 (C) nuclear cell lysate (35 μg protein in RIPA buffer). (CK1 epsilon 抗体 (C-Term))

ABIN238540 (1µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). (CK1 epsilon 抗体 (C-Term))

ABIN238540 (5µg/ml) staining of paraffin embedded Human Tonsil. (CK1 epsilon 抗体 (C-Term))

IHC

ABIN238540 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (CK1 epsilon 抗体 (C-Term))

ABIN238540 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. (CK1 epsilon 抗体 (C-Term))

ABIN238540 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0. (CK1 epsilon 抗体 (C-Term))

FACS

6 images

产品编号 ABIN238540

发货至: 中国

Datasheet as PDF

抗原 See all CK1 epsilon (CSNK1E) 抗体

CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))
抗原表位
13

5

5

5

5

5

4

4

3

2

2

2

1

1

1

1

1

1

1
C-Term
适用
63

38

18

3

3

2

2

2

2

2

2

2

1

1

1

1

1
人
宿主
61

7

2
山羊
Compatible Secondaries
克隆类型
63

7
多克隆
标记
38

6

6

4

2

2

2

2

2

2

2

2
This CK1 epsilon antibody is un-conjugated
应用范围
47

32

30

13

11

5

3

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

原理

CSNK1E

序列

PASQTSVPFD HLGK

交叉反应

犬, 人, 小鼠, 大鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

质量等级

Verified

免疫原

Peptide with sequence C-PASQTSVPFDHLGK, from the C Terminus of the protein sequence according to NP_001885.1, NP_689407.1.

亚型

IgG
anti-Casein Kinase 1, epsilon (CSNK1E) antibody
CSNK1E 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Casein Kinase 1, epsilon (CSNK1E) (Internal Region) antibody
CSNK1E 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Casein Kinase 1, epsilon (CSNK1E) (AA 247-416) antibody
CSNK1E 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Casein Kinase 1, epsilon (CSNK1E) (AA 17-231), (Isoform epsilon) antibody
CSNK1E 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Casein Kinase 1, epsilon (CSNK1E) (AA 1-416), (Isoform epsilon) antibody
CSNK1E 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Casein Kinase 1, epsilon (CSNK1E) antibody
CSNK1E 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Casein Kinase 1, epsilon (CSNK1E) (AA 2-100) antibody
CSNK1E 适用: 人 ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2E1 unconjugated

anti-Casein Kinase 1, epsilon (CSNK1E) (AA 331-360), (C-Term) antibody
CSNK1E 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB0845 unconjugated

anti-Casein Kinase 1, epsilon (CSNK1E) (Center) antibody
CSNK1E 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

anti-Casein Kinase 1, epsilon (CSNK1E) (AA 276-325) antibody
CSNK1E 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.
Western Blot: Approx 48 kDa band observed in nuclear lysates of cell lines A431and HeLa, and approx 45 kDa in nuclear lysates of cell line K562 (calculated MW of 47.3 kDa according to NP_001276841.1). Recommended concentration: 0.3-1 μg/mL. Primary incubatio
Peptide ELISA: antibody detection limit dilution 1:32000.

说明

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
抗原

CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

别名

CSNK1E (CSNK1E 产品)

背景

CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE, MGC10398 , CKI-epsilon, OTTHUMP00000063258, OTTHUMP00000063263, casein kinase 1 epsilon, casein kinase I epsilon

基因ID

1454, 27373, 58822

NCBI登录号

NP_001276841

途径

Hedgehog Signaling, M Phase