Arylsulfatase A 抗体 (Internal Region)
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- 抗原 See all Arylsulfatase A (ARSA) 抗体
- Arylsulfatase A (ARSA)
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Arylsulfatase A antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- Arylsulfatase A
- 序列
- YDLSKDPGEN YN
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-YDLSKDPGENYN, from the internal region of the protein sequence according to NP_000478.2.
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5 μg/mL.
Western Blot: Approx 60-65Da band observed in Mouse and Rat Testis lysates (calculated MW of 53.7 kDa according to Mouse NP_033843.2 and Rat NP_001030105.2,). This molecular weight is routinely observed by other sources and was successfully blocked by inc
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Chronic ethanol consumption decreases serum sulfatide levels by suppressing hepatic cerebroside sulfotransferase expression in mice." in: Archives of toxicology, Vol. 88, Issue 2, pp. 367-79, (2014) (PubMed).
: "Hepatic Cerebroside Sulfotransferase Is Induced by PPAR? Activation in Mice." in: PPAR research, Vol. 2012, pp. 174932, (2012) (PubMed).
: "Chronic caloric restriction attenuates a loss of sulfatide content in PGC-1?-/- mouse cortex: a potential lipidomic role of PGC-1? in neurodegeneration." in: Journal of lipid research, Vol. 53, Issue 2, pp. 273-81, (2012) (PubMed).
: "Acute kidney injury induced by protein-overload nephropathy down-regulates gene expression of hepatic cerebroside sulfotransferase in mice, resulting in reduction of liver and serum sulfatides." in: Biochemical and biophysical research communications, Vol. 390, Issue 4, pp. 1382-8, (2009) (PubMed).
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Chronic ethanol consumption decreases serum sulfatide levels by suppressing hepatic cerebroside sulfotransferase expression in mice." in: Archives of toxicology, Vol. 88, Issue 2, pp. 367-79, (2014) (PubMed).
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- 抗原
- Arylsulfatase A (ARSA)
- 别名
- ARSA (ARSA 产品)
- 别名
- ARSA antibody, zgc:101575 antibody, arsa antibody, AS-A antibody, ASA antibody, AW212749 antibody, As-2 antibody, As2 antibody, TISP73 antibody, MLD antibody, mld antibody, arylsulfatase A antibody, arylsulfatase antibody, arylsulfatase A, gene 1 S homeolog antibody, ARSA antibody, arsa antibody, arsA antibody, RB6599 antibody, Arsa antibody, arsa.1.S antibody
- 背景
- ARSA, arylsulfatase A, MLD, cerebroside-sulfatase, cerebroside 3-sulfatase
- 基因ID
- 410, 11883, 315222
- NCBI登录号
- NP_000478
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