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- 抗原 See all HLA-DR products
- HLA-DR
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HLA-DR antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Species reactivity (tested):Human
- 产品特性
- Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- Thymocyte membrane
- 克隆位点
- MEM-12
- 亚型
- IgG1
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anti-HLA-DR (HLA-DR) antibody
HLA-DR 适用: 人 FACS, WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal TAL 1B5 unconjugated
anti-HLA-DR (HLA-DR) antibody (FITC)HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 FITC
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人 FACS, WB, IHC, IF, ISt, StM 宿主: 小鼠 Monoclonal SPM289 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人 FACS, WB, IP 宿主: 小鼠 Monoclonal MEM-12 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人, 犬, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal L243 unconjugated
anti-HLA-DR (HLA-DR) antibody (PE)HLA-DR 适用: 人, 犬, Non-Human Primate FACS 宿主: 小鼠 Monoclonal L243 PE
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal HL-39 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人, 犬, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC, Func 宿主: 小鼠 Monoclonal L243 unconjugated
anti-HLA-DR (HLA-DR) antibody (PerCP)HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 PerCP
anti-HLA-DR (HLA-DR) antibody (APC)HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 APC
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- 应用备注
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Flow Cytometry: Positive Control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Immunoprecipitation: Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Western Blotting (Non-Reducing Conditions): Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. Do not heat/boil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 with 15 mM sodium azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- HLA-DR
- 别名
- HLA Class II DR (HLA-DR 产品)
- 背景
- HLA DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of an alpha chain (36 kDa) and a beta chain (27 kDa). It is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response.Synonyms: HLA class II histocompatibility antigen DR, HLA-DR, MHC class II antigen DR
- 途径
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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