Suitable for use in ELISA (1:5.000-1:40.000) and Western blot (1:200-1:5.000). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute with 0.5 mL distilled water.
缓冲液
Lyophilized from 75 mM PBS, pH 7.2 containing 75 mM Sodium chloride, 0.5 mM EDTA and 15 mg/mL BSA, 0.02 % Sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Prepare working dilution only prior to immediate use.The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.Centrifuge product if not completely clear after standing for 10 minutes at room temperature. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.
储存条件
4 °C
储存方法
Store lyophilized product at 2-8 °C. After reconstitution, product is stable for several weeks at 2-8 °C as an undiluted liquid. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50 % followed by storage at -28 °C.