Reacts well with alpha-synuclein in Western blots and frozen and formalin-fixed/paraffin-embedded tissue sections. Recognizes human alpha-synuclein. Synuclein-alpha is the major component of Lewy bodies and Lewy neuritis in sporadic PD. dementia with Lewy Bodies and Lewy Body variant of AD. Lewy bodies are composed of truncated and phosphorylated intermediate neurofilament proteins, alpha synuclein, ubiquitin and associated enzymes. The synuclein phosphoproteins (15-20 kDa) are small highly conserved proteins in vertebrates. The synuclein family includes alpha and beta synucleins and loosely related gamma-synuclein and synoretin. The expression is abundant in neurons and typically localized at presynaptic terminals.
产品特性
MAb to Synuclein Alpha, Monoclonal antibody to Human Synuclein alpha, Tissue culture supernatant
Western/Dot blot (1:100 - 1:10.000) ELISA Immunohistochemistry: (1:100 - 1:1.000) frozen and formalin-fixed paraffin-embedded tissues (No formic acid, enzyme or HIER required). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
限制
仅限研究用
状态
Liquid
缓冲液
PBS.
储存液
Without preservative
注意事项
Avoid multiple freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.