RAB11A 抗体 (C-Term)
(1 validation)-
- 抗原 See all RAB11A 抗体
- RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RAB11A antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
- 交叉反应
- 犬, 人, 兔, 大鼠
- 交叉反应 （详细）
- Calculated cross reactivity: Ca Hu Rb Rt
- 产品特性
- Rab11A (RAB11A Member RAS Oncogene Family, Rab-11A, MGC1490, RAB11, Rab-11, Ras-related Protein Rab-11A, Ras-related protein Rab11A, YL8)
- 纯化方法
- Purified by immunoaffinity chromatography.
- 免疫原
- Synthetic peptide corresponding to the C-terminal hypervariable region of the human Rab11a sequence. Sequence Homology: Rab11b protein-10/17.
- 亚型
- IgG
- Top Product
- Discover our top product RAB11A Primary Antibody
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anti-RAB11A, Member RAS Oncogene Family (RAB11A) (C-Term) antibody
RAB11A 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, 绵羊, 猴, Pig, 小鸡, Hamster, 山羊, 驴, Cat, Avian WB 宿主: 山羊 Polyclonal unconjugated
anti-RAB11A, Member RAS Oncogene Family (RAB11A) (AA 1-216) antibodyRAB11A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F7-2A8 unconjugated
anti-RAB11A, Member RAS Oncogene Family (RAB11A) (C-Term) antibodyRAB11A 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, 绵羊, 猴, Pig, 小鸡, Hamster, 山羊, 驴, Cat, Avian WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-RAB11A, Member RAS Oncogene Family (RAB11A) antibodyRAB11A 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-RAB11A, Member RAS Oncogene Family (RAB11A) (AA 1-216) antibodyRAB11A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-RAB11A, Member RAS Oncogene Family (RAB11A) antibodyRAB11A 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-RAB11A, Member RAS Oncogene Family (RAB11A) antibodyRAB11A 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 6C8 unconjugated
anti-RAB11A, Member RAS Oncogene Family (RAB11A) antibodyRAB11A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAB11A, Member RAS Oncogene Family (RAB11A) (AA 1-216) antibodyRAB11A 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-RAB11A, Member RAS Oncogene Family (RAB11A) (C-Term) antibodyRAB11A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working conditions should be determined by the investigator.
- 限制
- 仅限研究用
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- by
- Epidermis Differentiation and Rheumatoid Autoimmunity (INSERM U1056 UDEAR), University of Toulouse
- No.
- #101022
- 日期
- 2017.07.27
- 抗原
- RAB11A
- Lot Number
- L16102562
- Method validated
- Western Blotting
- Positive Control
Cell lysates from total human epidermis
Cell lysates from reconstructed human epidermis transfected with control shRNA
- Negative Control
Cell lysates from reconstructed human epidermis transfected with RAB11A shRNA
- Notes
- Passed. ABIN2346690 specifically recognizes Rab11a in human epidermal samples.
- Primary Antibody
- ABIN2346690
- Secondary Antibody
- goat anti-rabbit Ig HRP-conjugated antibody (Southern Biotech, 4030-05, lot J1810)
- Full Protocol
- Obtain total human epidermis from abdominal dermolipectomy and treat with dispase (Sigma-Aldrich, D4693) to dissociate it from the dermis.
- For production of reconstructed human epidermis, obtain isolated keratinocytes from epidermal fragments. Grow cells in Epilife (Invitrogen Life Technologies, MEPICF500, lot 1838688) supplemented with penicillin and streptomycin (Sigma-Aldrich, P4333, lot 1868847), HKGS (Human Keratinocyte Growth Supplement, ThermoFisher Scientific, S0015, lot 1848268), 1.5mM calcium (Sigma-Aldrich 25011), KGF (Keratinocyte Growth Factor, Sigma-Aldrich, K1767, lot MKBX7825V) and vitamin C (Sigma-Aldrich, 49752) on polycarbonate culture inserts (Merck Millipore, PIHP01250) at 37°C and 5% CO2, according to Reynier et al. (2016).
- For silencing with interfering shRNA, transduce keratinocytes with MISSION pLKO.1 puro vector-based lentiviral particles containing a puromycin resistance gene and an shRNA insert under the human U6 promoter (Sigma-Aldrich, TRCN0000303291 for Rab11A and SHC002 for the negative control) following the manufacturer´s instructions, before cell seeding for production of reconstructed human epidermis.
- Lyse normal or reconstructed epidermis in 300µL/0.6cm2 1x Laemmli buffer (Tris-HCl 63mM pH6.8, SDS 2%, Glycerol 10%, Bromophenol blue 0.0005%, DTT 1mM, all Sigma-Aldrich) and heat at 95°C for 5min.
- Separate 5µL of total protein extracts in 1x Laemmli buffer on denaturing 10% polyacrylamide SDS gels in a Mini-Protean Tetra Cell electrophoresis cell system (Bio-Rad , 1658004) at 100V for 90min.
- Transfer proteins onto Hybond ECL membrane (GE Healthcare, RPN3032D) with a Mini TransBlot Western blotting system (Bio-Rad, 1658029) at 100V for 90 min.
- Block membrane with 1x PBS containing Tween 20 (Sigma-Aldrich) at 0.05% (PBST) and 5% low fat milk at 3% at RT for 1h.
- Incubate the membrane with primary rabbit anti-human RAB11A, Member RAS Oncogene Family (RAB11A) (C-Term) antibody (antibodies-online, ABIN2346690, lot L16102562) diluted 1:250 in blocking buffer ON at 4°C.
- Wash the membrane 3x for 15min with 1x PBST.
- Incubate the membrane with secondary goat anti-rabbit Ig HRP-conjugated antibody (Southern Biotech, 4030-05, lot J1810) diluted 1:10000 in blocking buffer for 90min at RT.
- Wash the membrane 3x for 15min with 1x PBST.
- Reveal protein bands using ECL prime Detection Reagent (GE Healthcare, RPN2238, lot 9838391) on a GBox apparatus (Syngene).
- Experimental Notes
生效 #101022 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Western blot analysis of reconstructed human epidermis cell lysates with ABIN2346690 reveals a band of the expected size for Rab11a in the lysates of the cells transfected with a scrambled control shRNA (left lane) which is absent in lysates from cells that were transfected with RAB11A shRNA. Total human epidermis lysates (right lane) served as control. The immunoblot with ABIN2346690 reveals the expected protein band.]()
Western blot analysis of reconstructed human epidermis cell lysates with ABIN2346690 reveals a band of the expected size for Rab11a in the lysates of the cells transfected with a scrambled control shRNA (left lane) which is absent in lysates from cells that were transfected with RAB11A shRNA. Total human epidermis lysates (right lane) served as control. The immunoblot with ABIN2346690 reveals the expected protein band.
Full Methods -
- 状态
- Liquid
- 缓冲液
- Supplied as a liquid in PBS, pH 7.4, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))
- 别名
- Rab11A (RAB11A 产品)
- 别名
- rab11 antibody, RAB11A antibody, wu:fi15h09 antibody, zgc:103679 antibody, yl8 antibody, Rab11a antibody, rab11A antibody, DDBDRAFT_0190819 antibody, DDBDRAFT_0191190 antibody, DDB_0190819 antibody, DDB_0191190 antibody, YL8 antibody, RAB11 antibody, RAB11A, member RAS oncogene family S homeolog antibody, RAB11A, member RAS oncogene family antibody, RAB11a, member RAS oncogene family antibody, Rab11 GTPase antibody, rab11A protein antibody, Rab11a, GTPase antibody, Rab GTPase antibody, rab11A, RAB family GTPase antibody, rab11a.S antibody, RAB11A antibody, rab11a antibody, Rab11a antibody, rab11A antibody
- 途径
- Regulation of Cell Size, Thromboxane A2 Receptor Signaling, Regulation of long-term Neuronal Synaptic Plasticity
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