CD15 (Granulocytes) 抗体 (Biotin)
适用: 人
FACS, IHC (f), IFA
宿主: 小鼠
Monoclonal
TG1
Biotin
-
- 抗原
- CD15 (Granulocytes)
- 适用
- 人
-
宿主
- 小鼠
- 克隆类型
- 单克隆
-
标记
- Biotin
- 应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Indirect Immunofluorescence Assay (IFA)
- 特异性
- Recognizes a (Mr 156 kDa) cell surface glycoprotein expressed on virtually all peripheral blood granulocytes.(1,2,3) The CD15 antigen is expressed on approximately 95% of human peripheral blood eosinophils and neutrophils and is present on approximately 20% of mature circulating monocytes. Anti-CD15 has virtually no reactivity with T or B cells.(1,2,3)
- 产品特性
- MAb to CD15 Granulocytes, Monoclonal antibody to CD15 (Granulocytes) Biotin conjugated
- 纯化方法
- Ion Exchange Chromatography and gel filtration. Tissue culture
- 免疫原
- Thymocyte glycoprotein fraction adsorbed to Con A and eluted with 0.1M glucose
- 克隆位点
- TG1
- 亚型
- IgM
-
-
- 应用备注
- Suitable for use in flow cytometry, IFA and IHC (acetone-fixed frozen sections). May be used for enumerating mature granulocytes in peripheral blood. We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 0.01 M PBS, pH7.2 containing 1 % BSA, 0.1 % Sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
DO NOT FREEZE.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
-
- 抗原
- CD15 (Granulocytes)
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