SIPA1 抗体 (N-Term)
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- 抗原 See all SIPA1 抗体
- SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))
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抗原表位
- N-Term
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SIPA1 antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 原理
- Sipa1 Antibody
- 交叉反应 (详细)
- This antibody is specific for mouse Sipa1 protein.
- 产品特性
- Synonyms: rabbit anti-Sipa1 antibody, Sipa-1, Sipa 1, GTPase activating protein Spa 1 antibody, p130 SPA-1 antibody, Signal induced proliferation associated 1 antibody, SPA1
- 纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of mouse Sipa1.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product SIPA1 Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: 1.25-2.5 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.25 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))
- 别名
- Sipa1 (SIPA1 产品)
- 背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Sipa1 (signal-induced proliferation associated gene 1) is a mitogen-induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. Sipa1 is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.
- 基因ID
- 20469
- UniProt
- P46062
- 途径
- Response to Water Deprivation
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