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ATR 抗体 (Internal Region)

Name: ATR 抗体 (Internal Region)
SKU: ABIN233834
Availability: OutOfStock

Rockland 600-401-a30 ATR 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated

Western blot using anti-ATR antibody shows detection of ATR in HeLa cell nuclear extract (lane 1). (ATR 抗体 (Internal Region))

1 image

产品编号 ABIN233834

Supplier Product No.: 600-401-a30

发货至: 中国

Datasheet as PDF

抗原 See all ATR 抗体

ATR (ATR serine/threonine kinase (ATR))
抗原表位
11

7

6

3

2

2

2

1

1

1

1

1

1

1

1

1

1
Internal Region
适用
45

6

5

1

1

1

1

1

1
人
宿主
39

5

2
兔
Compatible Secondaries
克隆类型
42

4
多克隆
标记
30

3

3

2

1

1

1

1

1

1

1

1
This ATR antibody is un-conjugated
应用范围
26

20

20

4

4

3

1

1

1

1
ELISA, Western Blotting (WB)

Supplier Product No.

600-401-a30

Supplier

Rockland

原理

ATR Antibody

交叉反应（详细）

This antibody is specific for human ATR protein.

产品特性

Synonyms: rabbit anti-ATR antibody, Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, ACTR antibody, AIB1 antibody, amplified in breast cancer 1 antibody, CAGH16 antibody, CBP interacting protein antibody, CTG26 antibody, FRP1, FRP-1, FRP 1

纯化方法

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

过滤

Sterile filtered

免疫原

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
Immunogen Type: Conjugated Peptide

亚型

IgG

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ATR 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

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ATR 适用: 人 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated

应用备注

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 301 kDa in size corresponding to ATR by western blotting in the appropriate cell lysate or extract. Lysates from HeLa, U2OS, RAW264.7 or HEK293 cells are suggested for western blotting. ATR is ubiquitously expressed, with highest expression levels in the testes. Note that isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:15,000 - 1:70,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.28 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
抗原

ATR (ATR serine/threonine kinase (ATR))

别名

ATR (ATR 产品)

背景

Background: Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidyl-inositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during S-phase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.

基因ID

545, 157266317

UniProt

Q13535

途径

Positive Regulation of Response to DNA Damage Stimulus