RSL1D1 抗体
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- 抗原 See all RSL1D1 products
- RSL1D1 (Ribosomal L1 Domain Containing 1 (RSL1D1))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RSL1D1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-401-a24
- Supplier
- Rockland
- 原理
- PBK1 Antibody
- 交叉反应 （详细）
- This antibody is specific for human PBK1 protein.
- 产品特性
- Synonyms: rabbit anti-PBK1 antibody, PBK-1, PBK 1, CATX-11, CATX11 antibody, RSL1D1, Cellular senescence inhibited gene protein antibody, CSIG antibody, L12 antibody, Ribosomal L1 domain containing protein 1 antibody
- 纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human PBK1.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
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anti-Ribosomal L1 Domain Containing 1 (RSL1D1) (AA 1-490) antibody
RSL1D1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Ribosomal L1 Domain Containing 1 (RSL1D1) (AA 151-250) antibodyRSL1D1 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal L1 Domain Containing 1 (RSL1D1) (AA 455-485) antibodyRSL1D1 适用: 人 WB 宿主: 兔 Polyclonal RB54867 unconjugated
anti-Ribosomal L1 Domain Containing 1 (RSL1D1) (Internal Region) antibodyVerified RSL1D1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ribosomal L1 Domain Containing 1 (RSL1D1) (AA 401-450) antibodyRSL1D1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal L1 Domain Containing 1 (RSL1D1) (C-Term) antibodyRSL1D1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to PBK1 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous PBK1. For immunofluorescence microscopy, fix cells with methanol.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:2,500 - 1:10,000
IF Microscopy Dilution: 1:100
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- RSL1D1 (Ribosomal L1 Domain Containing 1 (RSL1D1))
- 别名
- RSL1D1 (RSL1D1 产品)
- 背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). PBK1 protein (also known as Ribosomal L1 domain-containing protein 1, cellular senescence-inhibited gene protein, and CATX-11) was isolated from highly invasive first trimester trophoblast cells and has been proposed to regulate their naturally occurring invasive behavior (Huch et al., 1998). PBK1 was also found to be over-expressed in non-small-cell lung cancer (NSCLC) cells (Petroziello et al., 2004). A recent study suggests that PBK1 may up-regulate the urokinase-type plasminogen activator (uPA) gene, which plays an important role in cellular matrix degradation and activation of other protease systems involved in cell invasion (Tong et al., 2005). The cellular localization dynamics of PBK1, as well as the data from its yeast homologue (Cic1p/Nsa3p) suggest that PBK1 is essential for ribosome biogenesis (Fatica et al., 2003). Thus, PBK1 could be controlling cell proliferation by regulating the level of ribosome production. It is hypothesized that PBK1 is involved in regulating both, cell proliferation and invasiveness, therefore playing a dual function in cancer cells transformation.
- 基因ID
- 26156, 62901068
- UniProt
- O76021
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