PIN1 抗体 (Internal Region)
(3 references)
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- 抗原 See all PIN1 抗体
- PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PIN1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Supplier Product No.
- 600-401-a20
- Supplier
- Rockland
- 原理
- PIN1 Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against human Pin1.
- 产品特性
- Synonyms: rabbit anti-PIN! antibody, PIN-1, PIN 1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody, Peptidyl-prolyl cis-trans isomerase Pin1, Prolyl isomerase antibody, Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody, Protein NIMA interacting 1 antibody, Rotamase Pin1 antibody, PPIase Pin1 antibody
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Pin1.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product PIN1 Primary Antibody
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anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 30-56) antibody
PIN1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 1-163) antibodyPIN1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 30-56) antibodyPIN1 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB22881 unconjugated
anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (N-Term) antibodyPIN1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (pSer16) antibodyPIN1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) antibodyPIN1 适用: 人, 小鼠, 大鼠, Green Monkey IHC (p), IF (cc) 宿主: 小鼠 Monoclonal 5F5 unconjugated
anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 2-163) antibodyPIN1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 1-163) antibodyPIN1 适用: 人 ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 1-163) antibodyPIN1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 64-163) antibodyPIN1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5A8 unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 5 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to Pin1 by western blotting in the appropriate cell lysate or extract. Lysates from 3T3, Jurkat, 293 or HeLa cells, as well as HeLa nuclear extract, are recommended for use as positive controls.
Western Blot Dilution: 1:500 - 1:3,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:2,500 - 1:10,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.2 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor α in breast cancer." in: PLoS ONE, Vol. 8, Issue 2, pp. e55355, (2013) (PubMed).
: "Androgen receptor serine 81 mediates Pin1 interaction and activity." in: Cell cycle (Georgetown, Tex.), Vol. 11, Issue 18, pp. 3415-20, (2013) (PubMed).
: "Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1." in: Cell death and differentiation, Vol. 19, Issue 7, pp. 1152-61, (2012) (PubMed).
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: "The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor α in breast cancer." in: PLoS ONE, Vol. 8, Issue 2, pp. e55355, (2013) (PubMed).
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- 抗原
- PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
- 别名
- PIN1 (PIN1 产品)
- 背景
- Background: Pin1 (peptidylprolyl cis/trans isomerase NIMA-interacting 1 protein) is an essential peptidylprolyl isomerase that regulates mitosis, presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Pin1 displays a preference for an acidic residue N-terminal to the isomerized proline bond and also catalyzes pSer/Thr-Pro cis/trans isomerizations. Pin1 shows a nuclear localization.
- 基因ID
- 5300, 5453898
- UniProt
- Q13526
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