TAF1 抗体 (C-Term)
(1 reference)
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- 抗原 See all TAF1 抗体
- TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))
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抗原表位
- C-Term
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TAF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
- Supplier Product No.
- 600-401-995
- Supplier
- Rockland
- 原理
- TAF1 Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against human TAF1 protein.
- 产品特性
- Synonyms: Rabbit anti-TAF1 antibody, rabbit anti-Anti-Transcription Initiation Factor TFIID Subunit 1 antibody, TAF-1, TAF 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, TAFII-250
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human TAF1.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
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anti-TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (C-Term) antibody
TAF1 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 兔, Pig WB, IHC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (AA 1784-1893) antibodyTAF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E11 unconjugated
anti-TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (AA 1784-1893) antibodyTAF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G9 unconjugated
anti-TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (Internal Region) antibodyTAF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (C-Term) antibodyTAF1 适用: 人, 犬, 马, Pig, 小鸡, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) antibodyTAF1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (AA 1800-1849) antibodyTAF1 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 兔, Pig, 小鸡, 猴, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (AA 1630-1893) antibodyTAF1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 10 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, ChIP, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 213 kDa in size corresponding to TAF1 protein by western blotting in the appropriate cell lysate or extract.
ChIP Dilution: User Optimized
Western Blot Dilution: 1:100 - 1:2,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:150,000 - 1:300,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.15 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells." in: Molecular and cellular biology, Vol. 32, Issue 10, pp. 1984-97, (2012) (PubMed).
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: "The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells." in: Molecular and cellular biology, Vol. 32, Issue 10, pp. 1984-97, (2012) (PubMed).
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- 抗原
- TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))
- 别名
- TAF1 (TAF1 产品)
- 背景
- Background: Anti-TAF1 antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors. TFIID nucleates the formation of transcription pre-initiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II. TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53. TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1). The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity. TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18 % of mammalian genes.
- 基因ID
- 6872
- NCBI登录号
- NP_001273003
- UniProt
- P21675
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