UBE2J1 抗体 (N-Term)
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- 抗原 See all UBE2J1 抗体
- UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This UBE2J1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Supplier Product No.
- 600-401-991
- Supplier
- Rockland
- 原理
- UBE2J1 Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against human Ube2j1 protein.
- 产品特性
- Synonyms: rabbit anti-UBE2J1 antibody, rabbit anti-E2 J1 antibody, rabbit anti-Ubiquitin conjugating enzyme E2J1 antibody, Non-canonical ubiquitin-conjugating enzyme 1, Ube2j1, E2 ubiquitin-conjugating enzyme J1, NCUBE-1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E, HsUBC6e
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product UBE2J1 Primary Antibody
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anti-Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1) antibody
UBE2J1 适用: 人 WB, IHC, IHC (p), FACS 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1) antibodyUBE2J1 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IHC, IHC (p), FACS 宿主: 小鼠 Monoclonal 3D1 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1) (AA 1-100) antibodyUBE2J1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1) (N-Term) antibodyUBE2J1 适用: 人, 小鼠, 大鼠, 豚鼠, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1) antibodyUBE2J1 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1) (AA 9-118) antibodyUBE2J1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6A12 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1) (N-Term) antibodyUBE2J1 适用: 人, 小鼠, 大鼠, 豚鼠, 猴, 兔, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1) antibodyUBE2J1 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1) antibodyUBE2J1 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1) (AA 9-118) antibodyUBE2J1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35-40 kDa in size corresponding to Ube2j1 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:200 - 1:2,000
Immunoprecipitation Dilution: 1-2 μg
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.71 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))
- 别名
- UBE2J1 (UBE2J1 产品)
- 背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal transmembrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.
- 基因ID
- 51465, 37577122
- UniProt
- Q9Y385
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