Tamalin/GRASP 抗体 (N-Term)
(4 references)
Quick Overview for Tamalin/GRASP 抗体 (N-Term) (ABIN233820)
抗原
See all Tamalin/GRASP (GRASP) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 600-401-988
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Supplier
- Rockland
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原理
- Tamalin Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against mouse Tamalin protein.
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产品特性
- Synonyms: rabbit anti-Tamalin antibody, rabbit anti-Grasp antibody, GRASP 1 antibody, GRP1-associated scaffold protein, GRIP associated protein 1 antibody, GRIP1 associated protein 1 antibody, GRIPAP 1 antibody, General receptor for phosphoinositides 1-associated scaffold protein
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse Tamalin protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-GRP1 (General Receptor For phosphoinositides 1)-Associated Scaffold Protein (GRASP) antibody
GRASP 适用: 小鼠, 大鼠, 人 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GRP1 (General Receptor For phosphoinositides 1)-Associated Scaffold Protein (GRASP) antibodyGRASP 适用: 小鼠, 大鼠, 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GRP1 (General Receptor For phosphoinositides 1)-Associated Scaffold Protein (GRASP) (AA 85-134) antibodyGRASP 适用: 小鼠, 大鼠, 人, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, Bat, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-GRP1 (General Receptor For phosphoinositides 1)-Associated Scaffold Protein (GRASP) (N-Term) antibodyGRASP 适用: 小鼠, 大鼠, 人, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-GRP1 (General Receptor For phosphoinositides 1)-Associated Scaffold Protein (GRASP) (C-Term) antibodyGRASP 适用: 小鼠, 大鼠, 人, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 42 kDa in size corresponding to Tamalin protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:3,000
Immunoprecipitation Dilution: 1 μg
ELISA Dilution: 1:4,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.03 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Hyperpolarization-activated, cyclic nucleotide-gated HCN2 cation channel forms a protein assembly with multiple neuronal scaffold proteins in distinct modes of protein-protein interaction." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 9, Issue 7, pp. 631-40, (2004) (PubMed).
: "Phosphorylation and recruitment of Syk by immunoreceptor tyrosine-based activation motif-based phosphorylation of tamalin." in: The Journal of biological chemistry, Vol. 279, Issue 31, pp. 32308-15, (2004) (PubMed).
: "Direct interaction and functional coupling between metabotropic glutamate receptor subtype 1 and voltage-sensitive Cav2.1 Ca2+ channel." in: The Journal of biological chemistry, Vol. 278, Issue 27, pp. 25101-8, (2003) (PubMed).
: "Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 4, pp. 1280-9, (2002) (PubMed).
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: "Hyperpolarization-activated, cyclic nucleotide-gated HCN2 cation channel forms a protein assembly with multiple neuronal scaffold proteins in distinct modes of protein-protein interaction." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 9, Issue 7, pp. 631-40, (2004) (PubMed).
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- Tamalin/GRASP (GRASP) (GRP1 (General Receptor For phosphoinositides 1)-Associated Scaffold Protein (GRASP))
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别名
- Grasp
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Tamalin, also named General receptor for phosphoinositides 1-associated scaffold protein (GRASP) is a PDZ (post-synaptic density protein/Drosophila disc large tumor suppressor/zo-1) domain-containing protein that interacts with group 1 metabotropic glutamate receptors (mGluRs). The PDZ domain-containing amino-terminal half of Tamalin binds directly to the class I PDZ-binding motif of group 1 mGluRs. The carboxyl-terminal half of Tamalin binds to cytohesins, which are guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. Tamalin forms a protein complex with group 1 mGluRs at the post-synaptic site of specific neuronal cells and serves as a key scaffold protein that links a complex formation between mGluR1a and cytohesins. It is reported that Tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1 mGluRs at synapses.
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基因ID
- 56149, 103472019
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UniProt
- Q9JJA9
抗原
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