Nuclear Factor kappa B p50 (NFkB p50) (N-Term) 抗体
(2 references)
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- 抗原
- Nuclear Factor kappa B p50 (NFkB p50)
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抗原表位
- N-Term
- 适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Gel Shift (GS)
- Supplier Product No.
- 100-4164s
- Supplier
- Rockland
- 原理
- NFkB p50 Antibody
- 交叉反应 （详细）
- Anti-Human NFkB p50 (NFKB1) may react non-specifically with other proteins.
- 产品特性
- Synonyms: rabbit Anti-NFKB p50 antibody, rabbit anti-NFKB1 antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
- 纯化方法
- This product was prepared from monospecific antiserum by delipidation and defibrination.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
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anti-Nuclear Factor kappa B p50 (NFkB p50) (N-Term) antibody
适用: 人 WB, ELISA, GS 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Gel Shift Dilution: 0.5 to 1.0 μL
Application Note: This product was tested by immunoblot and ELISA. Anti-Human NFkB p50 (NFKB1) is suitable for the detection by immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105, and was found to be reactive against Human NFkB p50 (NFKB1) at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. No reaction was observed against the analogous mouse protein. This product was also tested in a gel supershift assay and found to be reactive against p50:p50 homodimers and p:50:p65 heterodimers using 0.5 to 1.0 μL per assay.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: None
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "The eukaryotic translation initiation factor eIF4E is a direct transcriptional target of NF-κB and is aberrantly regulated in acute myeloid leukemia." in: Leukemia, Vol. 27, Issue 10, pp. 2047-55, (2013) (PubMed).
: "Nuclear factor-κB contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 25, pp. 9159-69, (2011) (PubMed).
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: "The eukaryotic translation initiation factor eIF4E is a direct transcriptional target of NF-κB and is aberrantly regulated in acute myeloid leukemia." in: Leukemia, Vol. 27, Issue 10, pp. 2047-55, (2013) (PubMed).
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- 抗原
- Nuclear Factor kappa B p50 (NFkB p50)
- 别名
- NFKB p50
- 背景
- Background: Anti NFkB p50 Antibody recognizes NFKB p50 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.
- 基因ID
- 4790
- UniProt
- P19838
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