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Mesothelin 抗体

Name: Mesothelin 抗体
SKU: ABIN233753
Availability: OutOfStock

Rockland 200-301-a88 MSLN 适用: 人 ELISA, WB, IHC, FACS 宿主: 小鼠 Monoclonal MN-1 unconjugated

Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. (Mesothelin 抗体)

IHC

Western blotting using anti-mesothelin antibodies to detect mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0. (Mesothelin 抗体)

Mesothelin expression in mesothelioma monolayers and spheroids. (Mesothelin 抗体)

FACS

3 images

(8 references)

产品编号 ABIN233753

Supplier Product No.: 200-301-a88

发货至: 中国

Datasheet as PDF

抗原 See all Mesothelin (MSLN) 抗体

Mesothelin (MSLN)
适用
97

18

5

2

2

2

1

1
人
宿主
78

25

4

1

1

1
小鼠
Compatible Secondaries
克隆类型
74

33

3
单克隆
标记
70

10

7

5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Mesothelin antibody is un-conjugated
应用范围
68

54

30

24

13

12

11

4

4

4

4

2

2

2

2

2

1

1

1

1
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Supplier Product No.

200-301-a88

Supplier

Rockland

原理

Mesothelin Antibody

交叉反应（详细）

This antibody is directed against human mesothelin protein.

产品特性

Synonyms: mouse anti-Mesothelin Antibody, Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen

纯化方法

This product was purified from tissue culture supernatant fluid by Protein A chromatography.

过滤

Sterile filtered

免疫原

Immunogen: This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
Immunogen Type: Recombinant Protein

克隆位点

MN-1

亚型

IgG2a

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应用备注

Flow Cytometry Dilution: 1:800
Immunohistochemistry Dilution: 1:100
Application Note: This antibody has been tested for use in immunohistochemistry, flow cytometry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1 % BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1 % BSA and reacted with tissue for 60 min at room temperature.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Zhang, Khanna, Jiang, Alewine, Miettinen, Pastan, Hassan: "Efficacy of Anti-mesothelin Immunotoxin RG7787 plus Nab-Paclitaxel against Mesothelioma Patient-Derived Xenografts and Mesothelin as a Biomarker of Tumor Response." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 23, Issue 6, pp. 1564-1574, (2018) (PubMed).

Oehl, Kresoja-Rakic, Opitz, Vrugt, Weder, Stahel, Wild, Felley-Bosco: "Live-Cell Mesothelioma Biobank to Explore Mechanisms of Tumor Progression." in: Frontiers in oncology, Vol. 8, pp. 40, (2018) (PubMed).

Khanna, Thomas, Abate-Daga, Zhang, Morrow, Steinberg, Orlandi, Ferroni, Schlom, Guadagni, Hassan: "Malignant Mesothelioma Effusions Are Infiltrated by CD3+ T Cells Highly Expressing PD-L1 and the PD-L1+ Tumor Cells within These Effusions Are Susceptible to ADCC by the Anti-PD-L1 Antibody Avelumab." in: Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, Vol. 11, Issue 11, pp. 1993-2005, (2017) (PubMed).

Awuah, Bera, Folivi, Chertov, Pastan: "Reduced Shedding of Surface Mesothelin Improves Efficacy of Mesothelin-Targeting Recombinant Immunotoxins." in: Molecular cancer therapeutics, Vol. 15, Issue 7, pp. 1648-55, (2017) (PubMed).

Xiang, Phung, Feng, Nagashima, Zhang, Broaddus, Hassan, Fitzgerald, Ho: "The development and characterization of a human mesothelioma in vitro 3D model to investigate immunotoxin therapy." in: PLoS ONE, Vol. 6, Issue 1, pp. e14640, (2011) (PubMed).

Feng, Zhang, Anver, Hassan, Ho: "In vivo imaging of human malignant mesothelioma grown orthotopically in the peritoneal cavity of nude mice." in: Journal of Cancer, Vol. 2, pp. 123-31, (2011) (PubMed).

Ho, Bera, Willingham, Onda, Hassan, FitzGerald, Pastan: "Mesothelin expression in human lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 5, pp. 1571-5, (2007) (PubMed).

Onda, Willingham, Nagata, Bera, Beers, Ho, Hassan, Kreitman, Pastan: "New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 11, Issue 16, pp. 5840-6, (2005) (PubMed).
抗原

Mesothelin (MSLN)

别名

MSLN (MSLN 产品)

背景

Background: Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol-anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71- kDa precursor protein that is processed to a 40- kDa glycosylphosphatidylinositol-anchored protein that composes the mature portion and an NH2 terminal 31- kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.

基因ID

10232

NCBI登录号

NP_005814

UniProt

Q13421

途径

EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process