Caspase 3 抗体 (N-Term)
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- 抗原 See all Caspase 3 (CASP3) 抗体
- Caspase 3 (CASP3)
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠, Cow, 犬, Pig, 兔, 豚鼠, 绵羊, 小鸡, Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Caspase 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- Recognizes human caspase-3. In apoptotic cells, this antibody also detects 20kD and 18kD proteins which correspond to the processed forms of caspase-3. Following the induction of apoptosis and the appearance of these smaller bands, a concurrent reduction in the amount of the procaspase enzyme is noted on immunoblot analysis. A weak ~30kD band may be detected in some samples. This band is not caspase-3 as it is detected in MCF7 cell lysates which lack the caspase-3 protein. Species cross-reactivity: mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, porcine, rabbit and yeast.
- 纯化方法
- Protein A purified
- 免疫原
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Recombinant full length human caspase-3 protein with an N-terminal (His) 6 tag.
Type of Immunogen: Recombinant protein - 亚型
- IgG
- Top Product
- Discover our top product CASP3 Primary Antibody
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- 应用备注
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Approved: WB (1 μg/mL)
Usage: Suitable for use in Western Blot. Western Blot (ECL): 1 μg/mL. A band is seen at ~36kD. Positive control: HeLa Nuclear Extract. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.2, 0.09 % sodium azide, 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- Caspase 3 (CASP3)
- 别名
- CASP3 / Caspase 3 (CASP3 产品)
- 背景
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Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease
Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama - 基因ID
- 836
- UniProt
- P42574
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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