Caspase 3 抗体 (N-Term)
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- 抗原 See all Caspase 3 (CASP3) 抗体
- Caspase 3 (CASP3)
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠, Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Caspase 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Recognizes cleaved human Caspase 3. Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases. Species cross-reactivity: mouse, bovine, and rat.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide corresponding residues adjacent to Asp175 in N-terminus of human caspase-3 (KLH coupled).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product CASP3 Primary Antibody
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- 应用备注
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Approved: ELISA, Flo (1:200), ICC, IF, IHC, IHC-Fr (1:200), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Recommend Dilution: Western Blot: 1:1000. Immunohistochemistry (Frozen): 1:200. Immunocytochemistry (IF):1:200. Flow Cytometry: 1:200.
Not recommended for: IHC-P - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
- 储存液
- Without preservative
- 注意事项
- avoid freeze thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- 抗原
- Caspase 3 (CASP3)
- 别名
- CASP3 / Caspase 3 (CASP3 产品)
- 背景
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Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease
Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama - 基因ID
- 836
- UniProt
- P42574
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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