Junctional Adhesion Molecule-A 1 (Extracellular Domain) 抗体
适用: 人
FACS, IHC (p), WB
宿主: 兔
Polyclonal
unconjugated
(4 references)-
- 抗原
- Junctional Adhesion Molecule-A 1
- 抗原表位
- Extracellular Domain
- 适用
- 人
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- 过滤
- 0.2 μm filtered
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- 应用备注
- For Western blot, flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Importance of junctional adhesion molecule-A for neointimal lesion formation and infiltration in atherosclerosis-prone mice." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 26, Issue 2, pp. e10-3, (2006) (PubMed).
: "Involvement of JAM-A in mononuclear cell recruitment on inflamed or atherosclerotic endothelium: inhibition by soluble JAM-A." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 4, pp. 729-35, (2005) (PubMed).
: "The functional interaction of the beta 2 integrin lymphocyte function-associated antigen-1 with junctional adhesion molecule-A is mediated by the I domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 10, pp. 6259-64, (2004) (PubMed).
: "JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes." in: Nature immunology, Vol. 3, Issue 2, pp. 151-8, (2002) (PubMed).
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: "Importance of junctional adhesion molecule-A for neointimal lesion formation and infiltration in atherosclerosis-prone mice." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 26, Issue 2, pp. e10-3, (2006) (PubMed).
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- 抗原
- Junctional Adhesion Molecule-A 1
- 背景
- The polyclonal antibody reacts with the first extracellular domain of human junction adhesion molecule (JAM)-A (also known as JAM, JAM-1 or F11R). Together with JAM-C (JAM-2) and JAM-B (VE-JAM or JAM-3), JAM-A belongs to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAMs are important for a variety of cellular processes, including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is expressed by endothelial and epithelial cells, platelets, neutrophils, monocytes, lymphocytes and erythrocytes. Like all other JAMs, JAM-A play an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-A also plays an important role in leukocyte transmigration. Leukocyte transmigration can be blocked by antibodies and by soluble JAM-A/Fc fusion proteins. Homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the b2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to actively guide leukocytes during transmigration. Several studies imply a role of JAM-A in the initiation of atherosclerosis, since JAM-A is upregulated on early atherosclerotic endothelium and adhesion of activated platelets on activated endothelium is mediated by homophilic interactions of JAM- A. The polyclonal antibody reacts with the 12 kDa extracellular domain 1 of the human JAM-A protein. The immunogen used for rabbit immunization is the extracellular domain of full-length human JAM-A. The antibody does not react with mouse JAM-A.
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