(5 references)-
- 抗原 See all CD73 (NT5E) 抗体
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD73 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 过滤
- 0.2 μm filtered
- 克隆位点
- 4G4
- Top Product
- Discover our top product NT5E Primary Antibody
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anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6B10 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibodyNT5E 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人 WB, FACS, IHC, ELISA, IF, StM, Coat 宿主: 小鼠 Monoclonal NT5E-2545 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人 WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal NT5E-2503 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibodyNT5E 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibodyNT5E 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6E3 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 520-550), (C-Term) antibodyNT5E 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02846 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)NT5E 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal AD2 PE
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibodyNT5E 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 151-250) antibodyNT5E 适用: 人, 小鼠, 大鼠, Cow, 犬, 绵羊 WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive HUVEC and fresh lymphocytes control Negative COS cells (Ref. 2) control
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.1 % bovine serum albumin.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
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: "IFN-alpha induced adenosine production on the endothelium: a mechanism mediated by CD73 (ecto-5'-nucleotidase) up-regulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 3, pp. 1646-53, (2004) (PubMed).
: "CD73 engagement promotes lymphocyte binding to endothelial cells via a lymphocyte function-associated antigen-1-dependent mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 10, pp. 5411-7, (2000) (PubMed).
: "Differential regulation and function of CD73, a glycosyl-phosphatidylinositol-linked 70-kD adhesion molecule, on lymphocytes and endothelial cells." in: The Journal of cell biology, Vol. 136, Issue 2, pp. 421-31, (1997) (PubMed).
: "CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1603-8, (1995) (PubMed).
: "Lymphocyte-vascular adhesion protein-2 is a novel 70-kDa molecule involved in lymphocyte adhesion to vascular endothelium." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 8, pp. 4228-38, (1993) (PubMed).
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: "IFN-alpha induced adenosine production on the endothelium: a mechanism mediated by CD73 (ecto-5'-nucleotidase) up-regulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 3, pp. 1646-53, (2004) (PubMed).
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- 抗原
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Abstract
- NT5E 产品
- 别名
- CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody
- 背景
- The monoclonal antibody 4G4 recognizes both membrane bound and soluble human CD73, also known as ecto-5'-nucleotidase. CD73 is a 70- kDa GPI-anchored cell surface molecule and belongs to the 5'-nucleosidase family. CD73 is useful as marker for lymphocyte differentiation. It is abundantly expressed on the vascular endothelium and at a low level on certain subpopulations of human lymphocytes. Like many glycosyl-phosphatidylinositol (GPI)-anchored molecules, it transmits potent activation signals in T cells when ligated by antibodies. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Ecto-5'-nucleotidase activity is an important mediator of the anti- inflammatory effect by converting extracellular AMP into a potent anti-inflammatory substance adenosine. CD73 has been shown to function as a co-stimulatory molecule in human T cells and to have a role in regulating lymphocyte adhesion. Triggering of CD73 on the surface of lymphocytes, but not on endothelial cells, results in the shedding of the CD73 and increased adhesion of lymphocytes to endothelium via LFA-1 clustering. Furthermore, CD73 has been implicated to mediate homing of skin-infiltrating lymphocytes in vivo. In B-cell chronic lymphocytic leukemia the expression of CD73 is decreased. Besides this, CD73 activity has been implicated as sensitive and useful indicator for mild zinc deficiency. The monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes, reduces enzyme activity, and inhibits the binding of lymphocytes to endothelial cells. Aliases Ecto-5'-nucleotidase, , EC3.1.3.5 Immunogen Inflamed synovial stroma from rheumatoid arthitis patients
- 途径
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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