(1 reference)-
- 抗原 See all Polymyxin B products
- Polymyxin B
- 适用
- 化学剂
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Polymyxin B antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 内毒素水平
- Low endotoxin level
- 克隆位点
- 45
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- 应用备注
- For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
- 限制
- 仅限研究用
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- 缓冲液
- 1 mL(> 200 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Product should be stored at -20 °C Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Polymyxin B-horseradish peroxidase conjugates as tools in endotoxin research." in: Analytical biochemistry, Vol. 207, Issue 2, pp. 311-6, (1993) (PubMed).
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: "Polymyxin B-horseradish peroxidase conjugates as tools in endotoxin research." in: Analytical biochemistry, Vol. 207, Issue 2, pp. 311-6, (1993) (PubMed).
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- 抗原
- Polymyxin B
- Abstract
- Polymyxin B 产品
- 物质类
- Chemical
- 背景
- The monoclonal antibody 45 reacts with Polymyxin B. The antibody binds to free Polymyxin B as well as to Polymyxin B already bound to LPS. The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo. Monoclonal antibody 45 enables the possibilities to study quantitatively the interaction of PMB and LPS.
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