(3 references)-
- 抗原
- Lipid A
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适用
- 化学剂
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB)
- 内毒素水平
- Low endotoxin level
- 克隆位点
- 43
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- 应用备注
- For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
- 限制
- 仅限研究用
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- 缓冲液
- 1 mL(> 200 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Product should be stored at -20 °C Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Endotoxin and CD14 in the progression of biliary atresia." in: Journal of translational medicine, Vol. 8, pp. 138, (2011) (PubMed).
: "The antibody reactivity of monoclonal lipid A antibodies is influenced by the acylation pattern of lipid A and the assay system employed." in: Immunobiology, Vol. 189, Issue 5, pp. 457-71, (1994) (PubMed).
: "Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A." in: Infection and immunity, Vol. 60, Issue 6, pp. 2201-10, (1992) (PubMed).
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: "Endotoxin and CD14 in the progression of biliary atresia." in: Journal of translational medicine, Vol. 8, pp. 138, (2011) (PubMed).
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- 抗原
- Lipid A
- 物质类
- Chemical
- 背景
- The monoclonal antibody 43 reacts with the hydrophilic backbone of free lipid A. This antibody only reacts with lipid A not covalent bound to LPS. LPS is chemically composed of a lipid part, lipid A, which is responsible for the endotoxic activities of LPS, and a heteropolysaccharide which generally consists of an oligosaccharide, termed the core region, and, in some bacterial families, of the so-called O chain, composed of repeating oligosaccharide units.
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