(1 reference)-
- 抗原 See all BLA-DQB products
- BLA-DQB (MHC Class II Antigen (BLA-DQB))
- 适用
- 小鼠
- 宿主
- 大鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
- 过滤
- 0.2 μm filtered
- 克隆位点
- ER-TR3
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- 应用备注
- For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Stromal cell types in the developing thymus of the normal and nude mouse embryo." in: European journal of immunology, Vol. 15, Issue 7, pp. 675-81, (1985) (PubMed).
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: "Stromal cell types in the developing thymus of the normal and nude mouse embryo." in: European journal of immunology, Vol. 15, Issue 7, pp. 675-81, (1985) (PubMed).
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- 抗原
- BLA-DQB (MHC Class II Antigen (BLA-DQB))
- 别名
- Major Histocompatibility Complex Class II (BLA-DQB 产品)
- 别名
- BoLA-DQB antibody, DQB antibody, DQB1 antibody, OLA-DQB antibody, MHC class II antigen antibody, BLA-DQB antibody
- 背景
- The monoclonal antibody ER-TR3 reacts with mouse major histocompatibitity complex class II (MHC class II), also known as I-A or I-E. MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen-presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they acquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to + CD4 T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC Class II. The monoclonal antibody ER-TR3 is a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells and macrophages) and for studying the development of T helper cells since they stain stromal cells in thymus. The level of antigen detected by ER-TR3 differs from strain to strain. The monoclonal antibody ER-TR3 cross reacts with human MHC class II on tonsil. Distribution of ER-TR3 among mouse strains with independent and recombinant haplotypes. Strain Haplotype
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