LY6C + LY6G 抗体 (FITC)
(2 references)-
- 抗原
- LY6C + LY6G
- 适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Immunoassay (IA)
- 过滤
- 0.2 μm filtered
- 克隆位点
- NIMP-R14
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anti-LY6C + LY6G antibody (PE)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE
anti-LY6C + LY6G antibody (Biotin)适用: 小鼠 FACS, IHC (p), Func, IA 宿主: 大鼠 Monoclonal NIMP-R14 Biotin
anti-LY6C + LY6G antibody适用: 小鼠 FACS, IHC, WB, Dep 宿主: 兔 Chimeric RB6-8C5 unconjugated Recombinant Antibody
anti-LY6C + LY6G antibody适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 unconjugated
anti-LY6C + LY6G antibody (APC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC
anti-LY6C + LY6G antibody (PE-Cy7)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy7
anti-LY6C + LY6G antibody (SPRD)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 SPRD
anti-LY6C + LY6G antibody (Biotin)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 Biotin
anti-LY6C + LY6G antibody (FITC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 FITC
anti-LY6C + LY6G antibody (APC-Cy7)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC-Cy7
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- 应用备注
- For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions. Positive Mouse neutrophils control Negative Mouse Thymocytes control
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 1.0 % BSA and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells." in: Parasite immunology, Vol. 9, Issue 6, pp. 667-82, (1988) (PubMed).
: "Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies." in: British journal of haematology, Vol. 57, Issue 3, pp. 489-94, (1984) (PubMed).
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: "Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells." in: Parasite immunology, Vol. 9, Issue 6, pp. 667-82, (1988) (PubMed).
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- 抗原
- LY6C + LY6G
- 背景
- The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre- monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. Aliases Lymphocyte antigen 6 complex locus protein G6c, myeloid differentiation antigen Gr1 Immunogen Purified BALB/c mouse neutrophils
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