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- 抗原 See all CD53 抗体
- CD53
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD53 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 产品特性
- 4AC53-2 monoclonal antibody reacts CD53, a 32-42 kDa molecule, which is expressed on thymocytes, T cells, B cells, NK cells, monocytes and granulocytes, but is not present on red blood cells, platelets and non-hematopoietic cells. CD53 cross-linking promotes activation of human B cells and rat macrophages, as well as signal transduction.This clone is cross reactive with non-human primate.
- 克隆位点
- 4AC53-2
- 亚型
- IgG2a
- Top Product
- Discover our top product CD53 Primary Antibody
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anti-CD53 (CD53) antibody
CD53 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7A8D3 unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1H2C7 unconjugated
anti-CD53 (CD53) antibody (PE)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 PE
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-53 unconjugated
anti-CD53 (CD53) (AA 100-180) antibodyCD53 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IF, IP, IHC 宿主: 兔 Chimeric HD77 unconjugated Recombinant Antibody
anti-CD53 (CD53) (Internal Region) antibodyCD53 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibody (FITC)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 FITC
anti-CD53 (CD53) (AA 65-160) antibodyCD53 适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, IF, Func 宿主: 兔 Chimeric 161-2 unconjugated Recombinant Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD53
- 别名
- CD53 (CD53 产品)
- 别名
- AI323659 antibody, Ox-44 antibody, Tspan25 antibody, MOX44 antibody, TSPAN25 antibody, OX44 antibody, CD53 antigen antibody, CD53 molecule antibody, Cd53 molecule antibody, Cd53 antibody, CD53 antibody
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