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- 抗原 See all ITGB1 抗体
- ITGB1 (Integrin beta 1 (ITGB1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ITGB1 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 产品特性
- 4ATS2/16.2.1 reacts with 130 kDa integrin b1 , which has a broad tissue distribution . It is expressed on lympnocytes , monocytes and weakly on granulovytes, but not on erythrocytes. On T cells, CD29 is more highly expressed on memory cells than naive cells. Integrin chain b asociated with integrin a subunits 1-6 ( CD49a-f) to form CD49/CD29 heterodimers that are involved in cell-cell and cell-matrix adhesion.It has been reported that CD29 is a critical molecule for embryogenesis and development. It also essential to the differentiation of hematopoietic stem cells and associated with tumor progression and metastasis.This clone is cross reactive with non-human primate.
- 克隆位点
- 4ATS2-16-2-1
- 亚型
- IgG1
- Top Product
- Discover our top product ITGB1 Primary Antibody
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anti-Integrin beta 1 (ITGB1) (AA 25-100) antibody
ITGB1 适用: 人, 小鼠, 大鼠, 绵羊, 兔 WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 1 (ITGB1) antibodyITGB1 适用: 人, 猴 WB, FACS, ELISA, IHC, DNA Mic 宿主: 小鼠 Monoclonal 3B6 unconjugated
anti-Integrin beta 1 (ITGB1) (AA 140-383) antibodyITGB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 1 (ITGB1) antibodyITGB1 适用: 人, Pig, 犬 WB, FACS, IP 宿主: 小鼠 Monoclonal MEM-101A unconjugated
anti-Integrin beta 1 (ITGB1) antibody (PE)ITGB1 适用: 人, Pig, 犬 FACS 宿主: 小鼠 Monoclonal MEM-101A PE
anti-Integrin beta 1 (ITGB1) (AA 297-380) antibodyITGB1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 1 (ITGB1) antibodyITGB1 适用: 人 WB, FACS, ELISA, IHC, IF 宿主: 兔 Monoclonal 32A10 unconjugated Recombinant Antibody
anti-Integrin beta 1 (ITGB1) (AA 454-714) antibodyITGB1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 1 (ITGB1) (AA 140-383) antibodyITGB1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Integrin beta 1 (ITGB1) (C-Term) antibodyITGB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- ITGB1 (Integrin beta 1 (ITGB1))
- 别名
- CD29 (ITGB1 产品)
- 别名
- CD29 antibody, FNRB antibody, GPIIA antibody, MDF2 antibody, MSK12 antibody, VLA-BETA antibody, VLAB antibody, 4633401G24Rik antibody, AA409975 antibody, AA960159 antibody, ENSMUSG00000051907 antibody, Fnrb antibody, Gm9863 antibody, gpIIa antibody, cd29 antibody, fnrb antibody, gpiia antibody, itgb1 antibody, mdf2 antibody, msk12 antibody, vla-beta antibody, vlab antibody, itgb1-a antibody, itgb1-b antibody, integrin subunit beta 1 antibody, integrin beta 1 (fibronectin receptor beta) antibody, integrin subunit beta 1 S homeolog antibody, integrin beta 1 antibody, integrin subunit beta 1 L homeolog antibody, ITGB1 antibody, Itgb1 antibody, itgb1.S antibody, LOC100009173 antibody, itgb1.L antibody
- 途径
- Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome
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