Vimentin 抗体
-
- 抗原 See all Vimentin (VIM) 抗体
- Vimentin (VIM)
-
适用
- 人, 大鼠, Pig, Cow, 小鸡, 猴, 犬, Cat
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Vimentin antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 特异性
- Recognizes a 57-60kD protein, human Vimentin. Shows no cross-reactivity with other closely related intermediate filament proteins including desmin and GFAP. Species cross-reactivity: monkey, bovine, canine, feline, chicken and rat. Does not recognize mouse.
- 纯化方法
- Purified
- 免疫原
-
Purified vimentin from porcine eye lens.
Type of Immunogen: Purified protein - 亚型
- IgG1
- Top Product
- Discover our top product VIM Primary Antibody
-
-
- 应用备注
-
Approved: ICC (5 - 10 μg/mL), IHC, IHC-P, WB (1:1000)
Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: 5-10 μg/mL, Can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antibody activity. Immunocytochemistry: 5-10 μg/mL. Positive control: Breast carcinoma tissue, colon, prostate, tonsil, tonsilar lymphoma, uterus and ureter. - 说明
-
Target Species of Antibody: Pig
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.2, 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
-
- 抗原
- Vimentin (VIM)
- 别名
- Vimentin (VIM 产品)
- 背景
-
Name/Gene ID: VIM
Synonyms: VIM, Vimentin - 基因ID
- 7431
- UniProt
- P08670
- 途径
- Caspase Cascade in Apoptosis
-