Vimentin 抗体
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- 抗原 See all Vimentin (VIM) 抗体
- Vimentin (VIM)
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适用
- 人, 大鼠, 小鸡
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Vimentin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Mesenchymal cells and mesenchymal derived tumors e. g. lymphoma, sarcoma and melanoma. Reacts with the 57kD protein corresponding to vimentin. Species cross-reactivity: Human, rat, chicken, potoroo.
- 纯化方法
- Protein G purified
- 免疫原
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Vimentin.
Type of Immunogen: Purified protein - 亚型
- IgG1
- Top Product
- Discover our top product VIM Primary Antibody
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- 应用备注
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Approved: Flo, ICC, IHC, IHC-Fr, IHC-P, WB (1:100 - 1:1000)
Usage: Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Immunocytochemistry. Immunohistochemistry (Frozen, Paraffin): 1:25-1:200 using ABC method. Paraffin sections require HIER with 10 mM citrate buffer, pH 6.0, for 10 min. Cool sections at room temperature for 20 min. Flow Cytometry: 1-25-1:200. Western Blot: 1:100-1:1000. Optimum dilutions to be determined by researcher. Hybridoma: PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from porcine lens. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.2, 0.1 % sodium azide. No stabilizing proteins added.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- 抗原
- Vimentin (VIM)
- 别名
- Vimentin (VIM 产品)
- 背景
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Name/Gene ID: VIM
Synonyms: VIM, Vimentin - 基因ID
- 7431
- UniProt
- P08670
- 途径
- Caspase Cascade in Apoptosis
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