PI3 Kinase p85 alpha Antibody detects endogenous levels of human PI3 Kinase p85 alpha. Predicted to react with mouse and rat PI3 Kinase p85 alpha according to sequence homology.Positive Control: Ramos
交叉反应 (详细)
PI3 Kinase p85 alpha Antibody detects endogenous levels of human PI3 Kinase p85 alpha. Predicted to react with mouse and rat PI3 Kinase p85 alpha according to sequence homology.Positive Control: Ramos
纯化方法
Immunoaffinity chromatography
免疫原
KLH-coupled synthetic peptide within AA 600-650 of human PI3 Kinase p85 alpha .
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western blot: 2-4 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther Applications: user optimized
限制
仅限研究用
状态
Lyophilized
缓冲液
PBS, pH 7.4, containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
储存条件
4 °C/-20 °C
储存方法
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Phosphoinositide 3-kinase (PI3K) catalyzes the phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit (p85). The catalytic subunits of PI3K include p110 alpha, p110 beta, p110 gamma, and p110 delta, while the regulatory subunits contain p85 alpha and p85 beta. p85 alpha regulates the catalytic activity of p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain. The p85 alpha has been implicated in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.PI3 Kinase p85 alpha Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 600-50 of human PI3 Kinase p85 alpha (Swiss Prot: P27986).