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- 抗原 See all CD247 抗体
- CD247 (CD247 Molecule (CD247))
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抗原表位
- Tyr153
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD247 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS)
- 特异性
- The mouse monoclonal antibody EM-17 recognizes phosphorylated intracellular tyrosine 153 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
- 交叉反应 （详细）
- Human, Mouse
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH
- 克隆位点
- EM-17
- 亚型
- IgG1
- Top Product
- Discover our top product CD247 Primary Antibody
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anti-CD247 Molecule (CD247) (Tyr72) antibody
CD247 适用: 人, 小鼠 WB, FACS, ICC 宿主: 小鼠 Monoclonal EM-26 unconjugated
anti-CD247 Molecule (CD247) (AA 1-164) antibodyCD247 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (AA 1-164) antibodyCD247 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated
anti-CD247 Molecule (CD247) (AA 88-153) antibodyCD247 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (AA 50-164) antibodyCD247 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) antibodyCD247 适用: 人 ELISA, FACS, IHC, ICC 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-CD247 Molecule (CD247) antibodyCD247 适用: 人 WB, FACS, IHC 宿主: 兔 Monoclonal unconjugated
anti-CD247 Molecule (CD247) (AA 1-165) antibodyCD247 适用: 人 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated
anti-CD247 Molecule (CD247) (AA 51-100) antibodyCD247 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (C-Term) antibodyCD247 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blotting: Recommended dilution: 2 - 5 μg/mL, positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended.
Flow cytometry: Intracellular staining, recommended dilution: 1-4 μg/mL, positive control: Jurkat cells treated with pervanadate. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze. Do not use after expiration date stamped on vial label.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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- 抗原
- CD247 (CD247 Molecule (CD247))
- 别名
- CD3 zeta (CD247 产品)
- 别名
- CD3-ZETA antibody, CD3H antibody, CD3Q antibody, CD3Z antibody, T3Z antibody, TCRZ antibody, 4930549J05Rik antibody, A430104F18Rik antibody, AW552088 antibody, Cd3 antibody, Cd3-eta antibody, Cd3-zeta antibody, Cd3h antibody, Cd3z antibody, Cd3zeta antibody, T3z antibody, Tcrk antibody, Tcrz antibody, LOC100136514 antibody, LOC100136549 antibody, LOC100305023 antibody, CD247 antibody, TCRzeta antibody, CD247 molecule antibody, CD247 antigen antibody, CD3zeta-2 antibody, CD3zeta-1 antibody, Cd247 molecule antibody, CD247 antibody, Cd247 antibody, cd3z antibody, cd247 antibody
- 背景
- CD247 Molecule,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 zeta chain, CD247, T3Z, TCRz, IMD25
- 基因ID
- 919
- UniProt
- P20963
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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