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Interferon gamma 抗体 (Biotin)

Name: Interferon gamma 抗体 (Biotin)
Brand: antibodies-online
SKU: ABIN1981857
Availability: OutOfStock

IFNG 适用: 人, Non-Human Primate WB, ELISA, FACS, IP, ICC, IHC (p) 宿主: 小鼠 Monoclonal 4S-B3 Biotin

Flow cytometry intracellular staining pattern of human peripheral whole blood (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S. (Interferon gamma 抗体 (Biotin))

FACS

ELISA

Flow cytometry multicolor intracellular staining pattern of human lymphocytes (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S. (Interferon gamma 抗体 (Biotin))

FACS

Separation of human IFN-gamma positive CD3 positive lymphocytes (red-filled) from IFN-gamma negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S. (Interferon gamma 抗体 (Biotin))

FACS

4 images

(4 references)

产品编号 ABIN1981857

发货至: 中国

Datasheet as PDF

抗原 See all Interferon gamma (IFNG) 抗体

Interferon gamma (IFNG)
适用
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人, Non-Human Primate
宿主
216

170

38

13

8

2

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2
小鼠
Compatible Secondaries
克隆类型
256

194

1
单克隆
标记
206

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This Interferon gamma antibody is conjugated to Biotin
应用范围
232

204

111

96

58

44

39

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1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

特异性

The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.

交叉反应（详细）

Human, Non-Human Primates

纯化方法

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

免疫原

Interferon gamma derived from human leukocytes

克隆位点

4S-B3

亚型

IgG1 kappa

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Microarray verified IFNG 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB393378 unconjugated

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IFNG 适用: 人, Non-Human Primate WB, ELISA, FACS, IP, ICC, IHC (p), RIA 宿主: 小鼠 Monoclonal 4S-B3 unconjugated

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anti-Interferon gamma (IFNG) (AA 23-156) antibody
IFNG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C8 unconjugated

anti-Interferon gamma (IFNG) (AA 24-166) antibody
IFNG 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D4 unconjugated

应用备注

Flow cytometry: Recommended dilution 1-4 μg/mL. Intracellular staining.
ELISA: Detection antibody in combination with capture antibody NIB42.

说明

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze. Do not use after expiration date stamped on vial label.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Brincks, Kucaba, Legge, Griffith: "Influenza-induced expression of functional tumor necrosis factor-related apoptosis-inducing ligand on human peripheral blood mononuclear cells." in: Human immunology, Vol. 69, Issue 10, pp. 634-46, (2008) (PubMed).

Janke, Witsch, Mages, Hutloff, Kroczek: "Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells." in: Immunology, Vol. 118, Issue 3, pp. 353-60, (2006) (PubMed).

Coles, Wing, Smith, Coraddu, Greer, Taylor, Weetman, Hale, Chatterjee, Waldmann, Compston: "Pulsed monoclonal antibody treatment and autoimmune thyroid disease in multiple sclerosis." in: Lancet (London, England), Vol. 354, Issue 9191, pp. 1691-5, (1999) (PubMed).

Caulfield, Fernandez, Sousa, Lane, Lee, Hawrylowicz: "Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte-macrophage colony-stimulating factor in the presence of glucocorticoids." in: Immunology, Vol. 98, Issue 1, pp. 104-10, (1999) (PubMed).
抗原

Interferon gamma (IFNG)

别名

IFN-gamma (IFNG 产品)

别名

IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody

背景

Interferon gamma,The interferon gamma (IFN-gamma, 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12, its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers, 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.,Interferon gamma, IFN-gamma

基因ID

3458

UniProt

P01579

途径

Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy