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CD3 抗体 (Biotin)

Name: CD3 抗体 (Biotin)
Brand: antibodies-online
SKU: ABIN1981855
Availability: OutOfStock

CD3 适用: 小鼠 FACS, IP, IHC (fro), ICC 宿主: Armenian Hamster Monoclonal 145-2C11 Biotin

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) Biotin antibody (concentration in sample 8 μg/mL, Streptavidin APC). (CD3 抗体 (Biotin))

FACS

Separation of murine CD3 positive splenocytes (red-filled) from CD3 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) Biotin antibody (concentration in sample 8 μg/mL, Streptavidin APC). (CD3 抗体 (Biotin))

FACS

2 images

(10 references)

产品编号 ABIN1981855

发货至: 中国

Datasheet as PDF

抗原 See all CD3 抗体

CD3
适用
178

93

24

20

14

10

8

8

7

5

3

3

2

1
小鼠
宿主
210

44

19

12

8
Armenian Hamster
克隆类型
283

6
单克隆
标记
88

36

30

23

22

11

8

6

6

5

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is conjugated to Biotin
应用范围
266

81

56

52

44

37

30

22

10

7

7

6

4

3

2

2

2

2

2

2

2

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

特异性

The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.

交叉反应（详细）

Mouse

纯化方法

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

免疫原

Mouse BM10-37 cytotoxic T lymphocytes

克隆位点

145-2C11

亚型

IgG kappa

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CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (Biotin)
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anti-CD3 (CD3) antibody (APC)
CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 APC

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CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (PE)
CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PE

应用备注

Flow cytometry: Recommended dilution: 1-2 μg/mL.

说明

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze. Do not use after expiration date stamped on vial label.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Han, Murray-Segal, Gershenzon, Zhang, Hodder, Pietersz, Mottram: "Idarubicin-145-2C11-F(ab')2 promotes peripheral tolerance and reduces chronic vascular disease in mouse cardiac allografts." in: Transplant immunology, Vol. 7, Issue 4, pp. 207-13, (2000) (PubMed).

Kearse: "Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14152-7, (1998) (PubMed).

Kinnaert, Pradier, Bournonville, Habrant, Goldman, Van Geertruyden et al.: "Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal ..." in: Transplant international : official journal of the European Society for Organ Transplantation, Vol. 9, Issue 4, pp. 386-91, (1997) (PubMed).

Henrickson, Reid, Bellet, Sawchuk, Hirsch: "Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11)." in: Transplantation, Vol. 60, Issue 8, pp. 828-35, (1995) (PubMed).

Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, (1995) (PubMed).

Jacobs, Vandeputte, Tolkamp, de Vries, Borst, Berns: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." in: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, (1994) (PubMed).

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, (1994) (PubMed).

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, (1991) (PubMed).

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, (1987) (PubMed).

Samelson, OShea, Luong, Ross, Urdahl, Klausner, Bluestone: "T cell antigen receptor phosphorylation induced by an anti-receptor antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 8, pp. 2708-14, (1987) (PubMed).
抗原

CD3

别名

CD3 (CD3 产品)

别名

CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody

背景

CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

基因ID

12501

UniProt

P22646

途径

TCR Signaling, Ubiquitin Proteasome Pathway