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P-Selectin 抗体 (Biotin)

Name: P-Selectin 抗体 (Biotin)
Brand: antibodies-online
SKU: ABIN1981854
Availability: OutOfStock

SELP 适用: 人, Non-Human Primate WB, FACS, IP, ICC 宿主: 小鼠 Monoclonal AK4 Biotin

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD62P (AK4) biotin antibody (concentration in sample 5 μg/mL, Streptavidin APC). (P-Selectin 抗体 (Biotin))

FACS

Separation of human CD62P positive thrombocytes (red-filled) from CD62P negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62P (AK4) biotin antibody (concentration in sample 5 μg/mL, Streptavidin APC). (P-Selectin 抗体 (Biotin))

FACS

2 images

(3 references)

产品编号 ABIN1981854

发货至: 中国

Datasheet as PDF

抗原 See all P-Selectin (SELP) 抗体

P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
适用
101

35

30

21

13

6

5

4

4

2

2

2

1
人, Non-Human Primate
宿主
67

48

1

1
小鼠
Compatible Secondaries
克隆类型
60

57
单克隆
标记
55

14

13

9

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This P-Selectin antibody is conjugated to Biotin
应用范围
83

58

28

27

26

23

13

13

12

12

10

9

8

2

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

特异性

The antibody AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes, it is relocated to the plasma membrane upon activation.

交叉反应（详细）

Human, Non-Human Primates

纯化方法

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

免疫原

Human platelets

克隆位点

AK4

亚型

IgG1 kappa

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anti-Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 198-432) antibody
SELP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 58-158) antibody
SELP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) antibody
SELP 适用: 人, Non-Human Primate WB, FACS, IP, ICC 宿主: 小鼠 Monoclonal AK4 unconjugated

anti-Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 30-200) antibody
SELP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 58-195) antibody
SELP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) antibody
SELP 适用: 人, Non-Human Primate WB, FACS, IP, ICC, Func 宿主: 小鼠 Monoclonal AK4 unconjugated

anti-Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (N-Term) antibody
SELP 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 198-432) antibody
SELP 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C9 unconjugated

anti-Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-771) antibody
SELP 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM150 unconjugated

应用备注

Flow cytometry: Recommended dilution: 2-5 μg/mL.

说明

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze. Do not use after expiration date stamped on vial label.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Ludwig, Schultz, Boehncke, Podda, Tandi, Krombach, Baatz, Kaufmann, von Andrian, Zollner: "Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules." in: The Journal of investigative dermatology, Vol. 122, Issue 3, pp. 830-6, (2004) (PubMed).

Kowalska, Ratajczak, Hoxie, Brass, Gewirtz, Poncz, Ratajczak: "Megakaryocyte precursors, megakaryocytes and platelets express the HIV co-receptor CXCR4 on their surface: determination of response to stromal-derived factor-1 by megakaryocytes and platelets." in: British journal of haematology, Vol. 104, Issue 2, pp. 220-9, (1999) (PubMed).

Dunlop, Skinner, Bendall, Favaloro, Castaldi, Gorman, Gamble, Vadas, Berndt: "Characterization of GMP-140 (P-selectin) as a circulating plasma protein." in: The Journal of experimental medicine, Vol. 175, Issue 4, pp. 1147-50, (1992) (PubMed).
抗原

P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

别名

CD62P (SELP 产品)

别名

PSELECT antibody, CD62 antibody, CD62P antibody, GMP140 antibody, GRMP antibody, LECAM3 antibody, PADGEM antibody, PSEL antibody, GMP-140 antibody, Grmp antibody, selectin P antibody, selectin, platelet antibody, Selp antibody, SELP antibody

背景

Selectin P,CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.,P-selectin, GMP140, SELP, PADGEM, GRMP, LECAM3, PSEL

基因ID

6403

UniProt

P16109