Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
P62Dok antibody detects endogenous levels of total p62Dok protein.
纯化方法
Afinity Chromatography using epitope-specific immunogen.
免疫原
The antiserum was produced against synthesized non-phosphopeptide derived from Human p62Dok (aa 360~364) around the phosphorylation site of Tyrosine 362 (P-I-Yp-D-E).
Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody undiluted (in aliquots) at -20 °C.
抗原
DOK1
(Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))
DOK1 (Downstream of tyrosine kinase 1, or p62Dok) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK1 appears to be a negative regulator of the insulin signaling pathway.Synonyms: Docking protein 1, Downstream of tyrosine kinase 1, p62(dok), pp62