APP 抗体 (AA 666-670)
(1 validation)-
- 抗原 See all APP 抗体
- APP (Amyloid beta (A4) Precursor Protein (APP))
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抗原表位
- AA 666-670
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This APP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- This antibody detects endogenous levels of total APP protein.
- 纯化方法
- Affinity Chromatography using epitope-specific immunogen.
- 免疫原
- peptide Sequence around amino acids 666~670 (A-V-T-P-E) derived from Human APP protein.
- Top Product
- Discover our top product APP Primary Antibody
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anti-Amyloid beta (A4) Precursor Protein (APP) (C-Term) antibody
APP 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (A4) Precursor Protein (APP) (AA 190-290) antibodyAPP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (A4) Precursor Protein (APP) (AA 18-270) antibodyAPP 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (A4) Precursor Protein (APP) (C-Term) antibodyAPP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (A4) Precursor Protein (APP) antibodyKO Validated APP 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Amyloid beta (A4) Precursor Protein (APP) (AA 381-480) antibodyAPP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), ICC, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (A4) Precursor Protein (APP) (pThr668) antibodyAPP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (A4) Precursor Protein (APP) (C-Term) antibodyAPP 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (A4) Precursor Protein (APP) (AA 18-286) antibodyAPP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (A4) Precursor Protein (APP) antibodyAPP 适用: 人 WB, ELISA, IHC, IF 宿主: 小鼠 Monoclonal 5A11E5 unconjugated
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- 应用备注
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- by
- Molecular Pathology Core
- No.
- #029576
- 日期
- 2014.01.11
- 抗原
- Lot Number
- 8715
- Method validated
- Immunofluorescence
- Positive Control
- Human brain
- Negative Control
- Human liver
- Notes
- Signal was detected in positive control tissue, and no signal was seen in negative control tissue.
- Primary Antibody
- Antibody: Amyloid beta A4 precursor protein (APP)
- Catalog number: ABIN197433
- Lot number: 8715
- Secondary Antibody
- Antibody: AlexaFluor 488 goat anti-Rabbit IgG
- Lot number: 702323
- Full Protocol
- Sections were deparaffinized and rehydrated.
- Sections were heated to 98°C for 20 min in citrate buffer pH 6.0 (Biogenex, HK086-9K) for antigen retrieval and cooled down for 20 min on the bench.
- Sections were blocked in 10% NGS (normal goat serum) for 20 min at room temperature.
- Sections were washed x 2 in 1xTBS buffer.
- Sections were incubated with primary antibody diluted 1:100 in Antibody Diluent (Invitrogen, 003218) at 4°C overnight.
- Sections were washed x 2 in 1xTBS buffer.
- Sections were incubated with AlexaFluor 488 Goat anti-Rabbit IgG 1:1000 for 60 min.
- Sections were washed x 3 in 1xTBS buffer.
- Sections were mounted with DAPI (Invitrogen, Prolong Gold antifade reagent with DAPI) and coverslipped.
- Sections were photographed with a Zeiss Axioskop2 microscope
- Experimental Notes
- None
生效 #029576 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1. Micrograph image of positive control (human brain FFPE tissue). APP staining appears in green.]()
Figure 1. Micrograph image of positive control (human brain FFPE tissue). APP staining appears in green.
![Figure 2: micrograph image of negative control (human liver FFPE tissue) stained with APP antibody.]()
Figure 2: micrograph image of negative control (human liver FFPE tissue) stained with APP antibody.
![Figure 3: micrograph image of isotype control (rabbit IgG isotype control antibody on human brain FFPE tissue).]()
Figure 3: micrograph image of isotype control (rabbit IgG isotype control antibody on human brain FFPE tissue).
![Figure 4: micrograph image of secondary antibody only control (no primary antibody on human brain FFPE tissue).]()
Figure 4: micrograph image of secondary antibody only control (no primary antibody on human brain FFPE tissue).
Full Methods -
- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody (in aliquots) at -20 °C.
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- 抗原
- APP (Amyloid beta (A4) Precursor Protein (APP))
- 别名
- Amyloid beta A4 Protein / APP (APP 产品)
- 别名
- AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, APPI antibody, CTFgamma antibody, CVAP antibody, PN-II antibody, PN2 antibody, aaa antibody, abeta antibody, abpp antibody, ad1 antibody, appi antibody, ctfgamma antibody, cvap antibody, pn2 antibody, APP antibody, APP-like antibody, APPL antibody, Abeta antibody, BcDNA:GH04413 antibody, CG7727 antibody, Dmel\\CG7727 antibody, EG:65F1.5 antibody, appl antibody, Abpp antibody, Adap antibody, Ag antibody, Cvap antibody, E030013M08Rik antibody, betaApp antibody, app antibody, wu:fj34d10 antibody, wu:fk65e12 antibody, zgc:85740 antibody, amyloid beta precursor protein antibody, amyloid beta (A4) precursor protein antibody, beta amyloid protein precursor-like antibody, amyloid beta (A4) precursor protein a antibody, amyloid beta precursor protein L homeolog antibody, APP antibody, app antibody, Appl antibody, App antibody, appa antibody, app.L antibody
- 背景
- Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver, ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri, moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II
- 基因ID
- 351
- NCBI登录号
- NP_000475
- UniProt
- P05067
- 途径
- Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour
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