This antibody AP02712PU detects endogenous levels of total Ezrin protein.
纯化方法
Immunoaffinity Chromatography using epitope-specific immunogen.
免疫原
The antiserum was produced against synthesized non-phosphopeptide derived from human Ezrin around the phosphorylation site of Threonine 566 (Y-K-Tp-L-R).
EZR
适用: 人
ELISA, IHC, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
Suitable for use in in Western blot (1/500-1/1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein which is enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cellsSynonyms: Cytovillin, EZR, VIL2, Villin-2, p81