DOK2 抗体 (pTyr299)
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- 抗原 See all DOK2 抗体
- DOK2
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抗原表位
- pTyr299
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DOK2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of p56Dok-2 only when phosphorylated at Tyrosine 299.
- 纯化方法
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
- 免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human p56Dok-2 around the phosphorylation site of tyrosine 299 (G-E-YP-A-V).
- Top Product
- Discover our top product DOK2 Primary Antibody
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anti-DOK2 (DOK2) (pTyr351) antibody
DOK2 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DOK2 (DOK2) (AA 123-412) antibodyDOK2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DOK2 (DOK2) antibodyDOK2 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DOK2 (DOK2) (Tyr419) antibodyDOK2 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DOK2 (DOK2) (C-Term) antibodyDOK2 适用: 人, Cow, Pig, 豚鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DOK2 (DOK2) (AA 380-412), (C-Term) antibodyDOK2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB1757 unconjugated
anti-DOK2 (DOK2) antibodyDOK2 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-DOK2 (DOK2) (pTyr139) antibodyDOK2 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DOK2 (DOK2) (pTyr299) antibodyDOK2 适用: 人, 小鼠, 猴 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-DOK2 (DOK2) (AA 1-260) antibodyDOK2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody (in aliquots) at -20 °C.
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- 抗原
- DOK2
- 别名
- DOK2 / p56 Dok2 (DOK2 产品)
- 别名
- DokR antibody, Frip antibody, Dok-2 antibody, p56DOK antibody, p56dok-2 antibody, docking protein 2 antibody, DOK2 antibody, Dok2 antibody
- 背景
- Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses using signal transduction. DOK2 acts as a multiple docking protein downstream of receptor or non-receptor tyrosine kinases. By this mechanism it acts to negatively regulate signal transduction and cell proliferation controlled by cytokines in a feedback loop. DOK2 is highly expressed in cells and tissues of hematopoietic origin as well as in lung. Expression of bcr/abl induces additional tyrosine phosphorylation of the DOK1 and DOK2 proteins and their association with Ras-GAP. Thus, it is suspected that DOK association regulates GAP activity toward Ras and that the DOK proteins serve as mediators of bcr-abl signaling. The role of DOK proteins in bcr-abl regulation may also be implicated in chronic myelogenous leukemia (CML), which is characterized by a Philadelphia chromosome translocation t(9,22). Such a mutation would result in a p210-bcr/abl chimeric protein-tyrosine kinase which has been found in many CML cases.Synonyms: Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), p56Dok-2
- 基因ID
- 9046
- NCBI登录号
- NP_003965
- UniProt
- O60496
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