Cortactin 抗体 (pTyr466)
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- 抗原 See all Cortactin (CTTN) 抗体
- Cortactin (CTTN)
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抗原表位
- pTyr466
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Cortactin antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 免疫原
- The antiserum was produced against synthesized phosphopeptide derived from mouse Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).
- Top Product
- Discover our top product CTTN Primary Antibody
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anti-Cortactin (CTTN) (N-Term) antibody
KO Validated CTTN 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 214-513) antibodyCTTN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) antibodyCTTN 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Cortactin (CTTN) (AA 341-450) antibodyCTTN 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2B5 unconjugated
anti-Cortactin (CTTN) (pTyr466) antibodyCTTN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) antibodyCTTN 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4C6 unconjugated
anti-Cortactin (CTTN) (AA 1-509) antibodyCTTN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 1-509) antibodyCTTN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (pTyr421) antibodyCTTN 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (N-Term) antibodyVerified CTTN 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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- 抗原
- Cortactin (CTTN)
- 别名
- Cortactin (CTTN 产品)
- 背景
- Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1
- 基因ID
- 10090
- UniProt
- Q60598
- 途径
- MAPK Pathway
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